Deoxynivalenol Modulates the Viability, ROS Production and Apoptosis in Prostate Cancer Cells
Dominika Ewa Habrowska-Górczyńska,
Karolina Kowalska,
Kinga Anna Urbanek,
Kamila Domińska,
Agata Sakowicz,
Agnieszka Wanda Piastowska-Ciesielska
Affiliations
Dominika Ewa Habrowska-Górczyńska
Laboratory of Cell Cultures and Genomic Analysis, Department of Comparative Endocrinology, Medical University of Lodz, Zeligowskiego 7/9, 90-752 Lodz, Poland
Karolina Kowalska
Laboratory of Cell Cultures and Genomic Analysis, Department of Comparative Endocrinology, Medical University of Lodz, Zeligowskiego 7/9, 90-752 Lodz, Poland
Kinga Anna Urbanek
Laboratory of Cell Cultures and Genomic Analysis, Department of Comparative Endocrinology, Medical University of Lodz, Zeligowskiego 7/9, 90-752 Lodz, Poland
Kamila Domińska
Department of Comparative Endocrinology, Medical University of Lodz, Zeligowskiego 7/9, 90-752 Lodz, Poland
Agata Sakowicz
Department of Medical Biotechnology, Medical University of Lodz, Zeligowskiego 7/9, 90-752 Lodz, Poland
Agnieszka Wanda Piastowska-Ciesielska
Laboratory of Cell Cultures and Genomic Analysis, Department of Comparative Endocrinology, Medical University of Lodz, Zeligowskiego 7/9, 90-752 Lodz, Poland
Deoxynivalenol (DON), known as vomitoxin, a type B trichothecene, is produced by Fusarium. DON frequently contaminates cereal grains such as wheat, maize, oats, barley, rye, and rice. At the molecular level, it induces ribosomal stress, inflammation and apoptosis in eukaryotic cells. Our findings indicate that DON modulates the viability of prostate cancer (PCa) cells and that the response to a single high dose of DON is dependent on the androgen-sensitivity of cells. DON appears to increase reactive oxygen species (ROS) production in cells, induces DNA damage, and triggers apoptosis. The effects of DON application in PCa cells are influenced by the mitogen-activated protein kinase (MAPK) and NFΚB- HIF-1α signaling pathways. Our results indicate that p53 is a crucial factor in DON-associated apoptosis in PCa cells. Taken together, our findings show that a single exposure to high concentrations of DON (2–5 µM) modulates the progression of PCa.
