Memorias do Instituto Oswaldo Cruz ()

Comparison of four molecular approaches to identify Candida parapsilosis complex species

  • Leonardo Silva Barbedo,
  • Maria Helena Galdino Figueiredo-Carvalho,
  • Mauro de Medeiros Muniz,
  • Rosely Maria Zancopé-Oliveira

DOI
https://doi.org/10.1590/0074-02760160412
Journal volume & issue
Vol. 112, no. 3
pp. 214 – 219

Abstract

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Since the description of Candida orthopsilosis and C. metapsilosis in 2005, several methods have been proposed to identify and differentiate these species from C. parapsilosis sensu stricto. Species-specific uniplex polymerase chain reaction (PCR) was performed and compared with sequencing of the D1/D2 region of the LSU 28S rDNA gene, microsatellite typing of C. parapsilosis sensu stricto, and PCR-restriction fragment length polymorphism patterns in the ITS1-5.8S-ITS2 region of the rDNA gene. There was agreement between results of testing of 98 clinical isolates with the four PCR-based methods, with 59 isolates identified as C. parapsilosis sensu stricto, 37 as C. orthopsilosis, and two as C. metapsilosis.

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