Screening and Validation of Leaf Width-Related Genes in Inbred Maize Lines
Shi Lu,
Qi Wang,
Junqi Yin,
Shubo Zheng,
Tingting Gao,
Xudong Zhou,
Jianxin Zhang,
Yuexian Xing,
Yingjie Ma,
Min Wang,
Delong Zhou,
Ming Lu,
Wenguo Liu,
Piwu Wang,
Zhijun Zhang
Affiliations
Shi Lu
Jilin Academy of Agricultural Sciences (Northeast Innovation Center of Agricultural Science and Technology in China), Shengtai Street, No.1363, Changchun 130033, China
Qi Wang
Jilin Academy of Agricultural Sciences (Northeast Innovation Center of Agricultural Science and Technology in China), Shengtai Street, No.1363, Changchun 130033, China
Junqi Yin
Jilin Academy of Agricultural Sciences (Northeast Innovation Center of Agricultural Science and Technology in China), Shengtai Street, No.1363, Changchun 130033, China
Shubo Zheng
Jilin Academy of Agricultural Sciences (Northeast Innovation Center of Agricultural Science and Technology in China), Shengtai Street, No.1363, Changchun 130033, China
Tingting Gao
Jilin Academy of Agricultural Sciences (Northeast Innovation Center of Agricultural Science and Technology in China), Shengtai Street, No.1363, Changchun 130033, China
Xudong Zhou
Jilin Academy of Agricultural Sciences (Northeast Innovation Center of Agricultural Science and Technology in China), Shengtai Street, No.1363, Changchun 130033, China
Jianxin Zhang
Jilin Academy of Agricultural Sciences (Northeast Innovation Center of Agricultural Science and Technology in China), Shengtai Street, No.1363, Changchun 130033, China
Yuexian Xing
Jilin Academy of Agricultural Sciences (Northeast Innovation Center of Agricultural Science and Technology in China), Shengtai Street, No.1363, Changchun 130033, China
Yingjie Ma
Jilin Academy of Agricultural Sciences (Northeast Innovation Center of Agricultural Science and Technology in China), Shengtai Street, No.1363, Changchun 130033, China
Jilin Academy of Agricultural Sciences (Northeast Innovation Center of Agricultural Science and Technology in China), Shengtai Street, No.1363, Changchun 130033, China
Ming Lu
Jilin Academy of Agricultural Sciences (Northeast Innovation Center of Agricultural Science and Technology in China), Shengtai Street, No.1363, Changchun 130033, China
Wenguo Liu
Jilin Academy of Agricultural Sciences (Northeast Innovation Center of Agricultural Science and Technology in China), Shengtai Street, No.1363, Changchun 130033, China
Piwu Wang
College of Agronomy, Jilin Agricultural University, Xincheng Street, No.1288, Changchun 130118, China
Zhijun Zhang
Jilin Academy of Agricultural Sciences (Northeast Innovation Center of Agricultural Science and Technology in China), Shengtai Street, No.1363, Changchun 130033, China
Leaf width is a key determinant of planting density and photosynthetic efficiency. In an effort to determine which genes regulate maize plant leaf width, we performed a genome-wide association study (GWAS) of 1.49 × 106 single nucleotide polymorphisms (SNPs) in 80 sequenced backbone inbred maize lines in Jilin Province, China, based upon phenotypic leaf width data from two years. In total, 14 SNPs were identified as being significantly related to leaf width (p Zm00001d044327 candidate gene. RNA was then isolated from 12 different inbred maize lines from this GWAS study cohort and was used to conduct qPCR analyses which revealed significant differences in Zm00001d044327 expression among strains exhibiting significant differences in leaf width. Based on an assessment of EMS mutant lines harboring a conserved amino acid stop mutation and two non-synonymous mutations in Zm00001d044327 that exhibited a narrow leaf width, these data suggested that Zm00001d044327 is a key regulator of maize leaf width.
