Improving Pepper Inbreds for Resistance to Pepper Yellow Leaf Curl Thailand Virus (PepYLCTHV) through Challenged Inoculations
Nattika Kumsee,
Patcharaporn Suwor,
Montinee Teerarak,
Wen-Shi Tsai,
Suchila Techawongstien,
Tanyarat Tarinta,
Sanjeet Kumar,
Nakarin Jeeatid,
Orawan Chatchawankanphanich,
Somsak Kramchote
Affiliations
Nattika Kumsee
School of Agricultural Technology, King Mongkut’s Institute of Technology Ladkrabang, Bangkok 10520, Thailand
Patcharaporn Suwor
School of Agricultural Technology, King Mongkut’s Institute of Technology Ladkrabang, Bangkok 10520, Thailand
Montinee Teerarak
School of Agricultural Technology, King Mongkut’s Institute of Technology Ladkrabang, Bangkok 10520, Thailand
Wen-Shi Tsai
Department of Plant Medicine, College of Agriculture, National Chiayi University, Chiayi 60004, Taiwan
Suchila Techawongstien
Department of Horticulture, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40000, Thailand
Tanyarat Tarinta
Department of Horticulture, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40000, Thailand
Sanjeet Kumar
Department of Genetics and Plant Breeding, Acharya Narendra Deva University of Agriculture & Technology, Ayodhya 224229, India
Nakarin Jeeatid
Department of Plant and Soil Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand
Orawan Chatchawankanphanich
Plant Virus and Bacteriophage Laboratory, National Center for Genetic Engineering and Biotechnology, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand
Somsak Kramchote
School of Agricultural Technology, King Mongkut’s Institute of Technology Ladkrabang, Bangkok 10520, Thailand
Chili peppers (Capsicum annuum L.) are an economically important crop worldwide. Pepper yellow leaf curl Thailand virus (PepYLCTHV), a Begomovirus causing yellow leaf mosaic disease of chili pepper, has been reported to incur 95% economic loss under epiphytotic conditions. Thirty-one chili genotypes were screened for resistance to PepYLCTHV disease through inoculation using 10–15 viruliferous whiteflies per plant. We purified two resistant lines (PEP6 and PEP12) through four generations of selfing and selection. At 28 days after inoculations, two chili genotypes (PEP6 and PEP12) had low disease severity and percentage of disease incidence (DI) compared to four susceptible checks, viz., Yodsonkeam80, Homsupan, Huareau12, and Pong Charian, which had a disease severity score of 5 with 100% DI. Thirty initial plants of PEP6 showed an average disease severity of 3.64 with 69.33% DI, and PEP12 showed an average disease severity of 3.83 with 77.67% DI. From these populations, we selected nine highly resistant plant of PEP6 and seven plants of PEP12 having a disease severity of 0 through pure-line selection for four selfing generations. The ratio of resistance (R) to susceptibility (S) consequently decreased. In PEP6, the ratio decreased from 1R:2S to 1R:1S, while in PEP12 the ratio decreased from 1R:3S to 1R:1S. These lines have potential for release as resistant lines for improving chili pepper resistance to PepYLCTHV and for developing makers associated with the resistant trait.
