Protective effect of Lanzhou lily polysaccharide fragment on X-ray-induced injury of bone marrow nucleated cells in mice

Abstract

This study focused on the radioprotective effects of Lanzhou lily polysaccharide fragment (LLP) on murine bone marrow nucleated cells (BMNCs) subjected to X-ray-induced injury. The lily polysaccharide extract was prepared using the ultrasonic auxiliary hot water method, followed by purification using ultrafiltration and Sephadex G-150 chromatography. The average molecular weight of the extract was 8 629.8 Da. Unlike the control and simple irradiation groups, the murine model of acute radioactive injury model was treated with different amounts of LLPs. Each group was evaluated for the number of BMNCs, micronucleus ratio, DNA injury, and cyclical changes. The results revealed increased injury in the simple irradiation group than in the LLP-treated group. The number of BMNCs increased by 45.5% (p<0.01) and 49.0% (p<0.01) in the medium-and high-dose groups, respectively. The micronucleus ratio reduced by 7.2% (p<0.05), 33.5% (p<0.01), and 59.9% in the low-, medium-, and high-dose groups, respectively. The tail moment and olive tail moment of the medium-and high-dose groups reduced by 51.2% (p<0.01), 58.2% (p<0.01), and 54.0% (p<0.01), and 60.5% (p<0.01), respectively. The fluorescence intensity of γH2AX reduced by 19.7% (p<0.01), 46.8% (p<0.01), and 61.0% (p<0.01) in the low-, medium-, and high-dose groups, respectively. The apoptosis rate reduced by 43.7% (p<0.01), 45.5% (p<0.01), and 54.6% (p<0.01) in the low-, medium-, and high-dose groups, respectively. The ratio of G0/G1 cells significantly decreased in the medium-and high-dose groups (p<0.01). The ratio of S-phase cells significantly increased in the medium-and high-dose groups (p<0.01), whereas the ratio of G2/M phase cells significantly increased in the high-dose group (p<0.01). Collectively, these results indicate that LLP extract is effective in alleviating X-ray-induced damage in BMNC, reduced micronucleus ratio, and protects against X-ray-induced double strand breaks (DSB) and apoptosis. Thus, LLP extract may play an important role radioprotection against X-rays-induced damage in BMNCs.

Keywords

• lanzhou lily polysaccharide fragment (llp)
• mice
• bone marrow nucleated cells (bmncs)
• x-rays
• radiation protection