Guoji laonian yixue zazhi (Mar 2024)
The Effect of Yishen Huoxue Tongqiao Method on the PI3K/AKT Signal Pathway andOxidative Stress in the Medial Vestibular Nucleus of D-galactose InducedAging Model Rats
Abstract
Objective To observe the effects of Yishen Huoxue Tongqiao method on the PI3K/AKT signaling pathway and oxidative stress expression level in the medial vestibular nucleus of aging model rats induced by intraperitoneal injection of D-galactose, and to explore the mechanism of Yishen Huoxue Tongqiao method in delaying the aging of the medial vestibular nucleus. Methods After adaptive feeding of 18 rats for 7 days, they were randomly divided into a blank group, a model group, and a Traditional Chinese Medicine group, with 6 rats in each group.The blank group was intraperitoneally injected with 500 mg/(kg·d) of physiological saline, while the model group and Traditional Chinese Medicine group were intraperitoneally injected with 500 mg/(kg·d) of D-galactose for 8 consecutive weeks.After successful modeling, the blank group and model group were orally administered physiological saline at 18.45 g/(kg·d), while the Traditional Chinese Medicine group was orally administered Yishen Huoxue Tongqiao decoction at 18.45 g/(kg·d) for 8 consecutive weeks.The vestibular function of each group of rats 2 hours after the last administration was evaluated, and the samples from the medial vestibular nucleus were collected.The expression levels of glutathione(GSH), malondialdehyde(MDA), reactive oxygen species(ROS) in each group were detected by the Elisa method, and the expression levels of PI3K and AKT1 in the medial vestibular nucleus were detected by the Western blot method. Results There was no statistically significant difference in the success rates of three sets of aerial righting reflex tests(P>0.05).The head deviation angle of the model group and the Traditional Chinese Medicine group was greater than that of the blank group, while the head deviation angle of the Traditional Chinese Medicine group was smaller than that of the model group, and the differences were statistically significant(P<0.05).Compared with the blank group, the expression of serum GSH in the model group decreased, while the expression of MDA and ROS increased.The expression levels of PI3K and AKT1 in the medial vestibular nucleus tissue decreased, and the differences were statistically significant(P<0.05).Compared with the model group, the expression levels of serum GSH and PI3K and AKT1 in the medial vestibular nucleus were increased in the Traditional Chinese Medicine group, while the expression levels of serum MDA and ROS were reduced, with statistical significance(P<0.05). Conclusion The Yishen Huoxue Tongqiao method has a protective effect on the medial vestibular nucleus of aging model rats, and its mechanism may be related to regulating the PI3K/AKT signaling pathway and oxidative stress.
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