Hsa_circ_0015326 Promotes the Proliferation, Invasion and Migration of Ovarian Cancer Through miR-127-3p/MYB

Abstract

Cuiying Zhang,1 Wei Liu,2 Fei Li,1 Yang Feng,1 Yunyun Li,1 Jia Wang3 1Department of Gynaecology, Yongchuan Hospital of Chongqing Medical University, Chongqing, People’s Republic of China; 2Department of Orthopedics, Yongchuan Hospital of Chongqing Medical University, Chongqing, People’s Republic of China; 3Department of Gynaecology and Obstetrics, University-Town Hospital of Chongqing Medical University, Chongqing, People’s Republic of ChinaCorrespondence: Jia WangDepartment of Gynaecology and Obstetrics, University-Town Hospital of Chongqing Medical University, No. 55 University Town Middle Road, Shapingba District, Chongqing, 401331, People’s Republic of ChinaTel [phone]Fax [phone]Email [email protected]: More and more evidences show that circular RNA (circRNA) has an important role in ovarian cancer (OC). Hsa_circ_0015326 is a newly discovered upregulated circRNA in OC, but its role and mechanism in OC have not been studied yet.Methods: Quantitative real-time PCR was used to detect the expression of hsa_circ_0015326, microRNA (miR)-127-3p and MYB. The viability, colony number, cell cycle process, invasion, migration and apoptosis of cells were determined using cell counting kit 8 assay, colony formation assay, flow cytometry, transwell assay and wound healing assay. Moreover, the protein expression levels of metastasis, proliferation, apoptosis markers and MYB were assessed using Western blot analysis. The interaction between miR-127-3p and hsa_circ_0015326 or MYB was confirmed by dual-luciferase reporter assay and RNA immunoprecipitation assay. Xenograft tumors were built to explore the role of hsa_circ_0015326 in OC tumor growth in vivo.Results: Elevated expression of hsa_circ_0015326 was identified in OC tissues and cells. Loss-of-function experiments suggested that silenced hsa_circ_0015326 inhibited the proliferation, invasion, migration, and promoted the apoptosis of OC cells in vitro, as well as inhibited OC tumorigenesis in vivo. Mechanically, hsa_circ_0015326 sponged miR-127-3p and miR-127-3p targeted MYB. The rescue experiments revealed that miR-127-3p inhibitor reversed the inhibitory effect of hsa_circ_0015326 silencing on OC progression, and MYB overexpression reversed the suppressive effect of miR-127-3p on OC progression. In addition, our data indicated that MYB expression was positively regulated by hsa_circ_0015326.Conclusion: This study showed that hsa_circ_0015326 could facilitate OC progression by regulating the miR-127-3p/MYB axis, which suggested that it might become a potential target for the treatment of OC.Keywords: ovarian cancer, hsa_circ_0015326, miR-127-3p, MYB

Keywords

• ovarian cancer
• hsa_circ_0015326
• mir-127-3p
• myb