Protective Effects of N-Acetylcysteine in Concanavalin A-Induced Hepatitis in Mice

Chengfen Wang; Yujing Xia; Yuanyuan Zheng; Weiqi Dai; Fan Wang; Kan Chen; Jingjing Li; Sainan Li; Rong Zhu; Jing Yang; Qin Yin; Huawei Zhang; Junshan Wang; Jie Lu; Yingqun Zhou; Chuanyong Guo

doi:10.1155/2015/189785

Mediators of Inflammation (Jan 2015)

Protective Effects of N-Acetylcysteine in Concanavalin A-Induced Hepatitis in Mice

Chengfen Wang,
Yujing Xia,
Yuanyuan Zheng,
Weiqi Dai,
Fan Wang,
Kan Chen,
Jingjing Li,
Sainan Li,
Rong Zhu,
Jing Yang,
Qin Yin,
Huawei Zhang,
Junshan Wang,
Jie Lu,
Yingqun Zhou,
Chuanyong Guo

Affiliations

Chengfen Wang: Department of Gastroenterology, Shanghai Tenth People’s Hospital, Tongji University of Medicine, Shanghai 200072, China
Yujing Xia: Department of Gastroenterology, Shanghai Tenth People’s Hospital, Tongji University of Medicine, Shanghai 200072, China
Yuanyuan Zheng: Department of Gastroenterology, Shanghai Tenth People’s Hospital, Tongji University of Medicine, Shanghai 200072, China
Weiqi Dai: Department of Gastroenterology, Shanghai Tenth People’s Hospital, Tongji University of Medicine, Shanghai 200072, China
Fan Wang: Department of Gastroenterology, Shanghai Tenth People’s Hospital, Tongji University of Medicine, Shanghai 200072, China
Kan Chen: Department of Gastroenterology, Shanghai Tenth People’s Hospital, Tongji University of Medicine, Shanghai 200072, China
Jingjing Li: Department of Gastroenterology, Shanghai Tenth People’s Hospital, Tongji University of Medicine, Shanghai 200072, China
Sainan Li: Department of Gastroenterology, Shanghai Tenth People’s Hospital, Tongji University of Medicine, Shanghai 200072, China
Rong Zhu: Department of Gastroenterology, Shanghai Tenth People’s Hospital, Tongji University of Medicine, Shanghai 200072, China
Jing Yang: Department of Gastroenterology, Shanghai Tenth People’s Hospital, Tongji University of Medicine, Shanghai 200072, China
Qin Yin: Department of Gastroenterology, Shanghai Tenth People’s Hospital, Tongji University of Medicine, Shanghai 200072, China
Huawei Zhang: Department of Gastroenterology, Shanghai Tenth People’s Hospital, Tongji University of Medicine, Shanghai 200072, China
Junshan Wang: Department of Gastroenterology, Shanghai Tenth People’s Hospital, Tongji University of Medicine, Shanghai 200072, China
Jie Lu: Department of Gastroenterology, Shanghai Tenth People’s Hospital, Tongji University of Medicine, Shanghai 200072, China
Yingqun Zhou: Department of Gastroenterology, Shanghai Tenth People’s Hospital, Tongji University of Medicine, Shanghai 200072, China
Chuanyong Guo: Department of Gastroenterology, Shanghai Tenth People’s Hospital, Tongji University of Medicine, Shanghai 200072, China

DOI: https://doi.org/10.1155/2015/189785
Journal volume & issue: Vol. 2015

Abstract

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This study was designed to study the protective effects and mechanisms of N-acetylcysteine (NAC) in concanavalin A-induced hepatitis in mice. In this study, pretreatment with NAC ameliorated the histopathological changes and suppressed inflammatory cytokines in ConA-induced hepatitis. The expression of IL-2, IL-6, TNF-α, and IFN-γ was significantly reduced in the NAC-treated groups. NAC activated PI3K/Akt pathway and inhibited the activation of NF-κB. Additionally, NAC reduced autophagosome formation, as assessed by detecting the expression of LC3 and Beclin 1. Our results demonstrate that NAC can alleviate ConA-induced hepatitis by regulating the PI3K/Akt pathway and reducing the late stages of autophagy. Our results described a new pharmaceutical to provide more effective therapies for immune hepatitis.

Published in Mediators of Inflammation

ISSN: 0962-9351 (Print); 1466-1861 (Online)
Publisher: Wiley
Country of publisher: United Kingdom
LCC subjects: Medicine: Pathology
Website: https://onlinelibrary.wiley.com/journal/4792

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