Discovery of tanshinone derivatives with anti-MRSA activity via targeted bio-transformation

Wenni He; Miaomiao Liu; Pei Huang; Wael M. Abdel-Mageed; Jianying Han; Jeramie D. Watrous; Don D. Nguyen; Wenzhao Wang; Fuhang Song; Huanqin Dai; Jingyu Zhang; Ronald J. Quinn; Tanja Grkovi; Houwei Luo; Lixin Zhang; Xueting Liu

doi:10.1016/j.synbio.2016.05.002

Synthetic and Systems Biotechnology (Sep 2016)

Discovery of tanshinone derivatives with anti-MRSA activity via targeted bio-transformation

Wenni He,
Miaomiao Liu,
Pei Huang,
Wael M. Abdel-Mageed,
Jianying Han,
Jeramie D. Watrous,
Don D. Nguyen,
Wenzhao Wang,
Fuhang Song,
Huanqin Dai,
Jingyu Zhang,
Ronald J. Quinn,
Tanja Grkovi,
Houwei Luo,
Lixin Zhang,
Xueting Liu

Affiliations

Wenni He: Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
Miaomiao Liu: Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
Pei Huang: Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
Wael M. Abdel-Mageed: Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia
Jianying Han: Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
Jeramie D. Watrous: Department of Chemistry & Biochemistry, University of California San Diego, La Jolla, CA 92093, United States
Don D. Nguyen: Department of Chemistry & Biochemistry, University of California San Diego, La Jolla, CA 92093, United States
Wenzhao Wang: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
Fuhang Song: Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
Huanqin Dai: Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
Jingyu Zhang: Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
Ronald J. Quinn: Eskitis Institute, Griffith University, Brisbane, QLD 4111, Australia
Tanja Grkovi: Eskitis Institute, Griffith University, Brisbane, QLD 4111, Australia
Houwei Luo: Department of Natural Products, China Pharmaceutical University, Nanjing 210009, China
Lixin Zhang: Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
Xueting Liu: Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China

DOI: https://doi.org/10.1016/j.synbio.2016.05.002
Journal volume & issue: Vol. 1, no. 3
pp. 187 – 194

Abstract

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Two potent anti-MRSA tanshinone glycosides (1 and 2) were discovered by targeted microbial biotransformation, along with rapid identification via MS/MS networking. Serial reactions including dehydrogenation, demethylations, reduction, glycosylation and methylation have been observed after incubation of tanshinone IIA and fungus Mucor rouxianus AS 3.3447. In addition, tanshinosides B (2) showed potent activities against serial clinical isolates of oxacillin-resistant Staphylococcus aureus with MIC values of 0.78 μg/mL. This is the first study that shows a significant increase in the level and activities of tanshinone glycosides relative to the substrate tanshinone IIA.

Published in Synthetic and Systems Biotechnology

ISSN: 2405-805X (Online)
Publisher: KeAi Communications Co., Ltd.
Country of publisher: China
LCC subjects: Technology: Chemical technology: Biotechnology; Science: Biology (General)
Website: https://www.keaipublishing.com/en/journals/synthetic-and-systems-biotechnology/

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