Scientific Reports (Jul 2024)

Synchrotron nano-FTIR spectroscopy for probing anticancer drugs at subcellular scale

  • Luis A. E. Batista de Carvalho,
  • Gianfelice Cinque,
  • Ana L. M. Batista de Carvalho,
  • Joana Marques,
  • Mark D. Frogley,
  • Hendrik Vondracek,
  • Maria Paula M. Marques

DOI
https://doi.org/10.1038/s41598-024-67386-y
Journal volume & issue
Vol. 14, no. 1
pp. 1 – 9

Abstract

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Abstract The cellular response to cisplatin was assessed in human osteosarcoma cells, using synchrotron-based (SR) Fourier Transform InfraRed nanospectroscopy (nano-FTIR) at the MIRIAM beamline B22 of Diamond Light Source (UK). This label-free mapping method delivered simultaneous morphological and biochemical information on a subcellular level (i.e. 100 s nanometer or better). Based on specific spectral biomarkers, the main biochemical constituents affected by the drug were identified at distinct locations within the cell´s inner body. Cisplatin was shown to have a noteworthy effect on proteins, mostly within the cytoplasm. A clear drug impact on cellular lipids was also observed. Within current literature on s-SNOM, this nanospectroscopy work represents a first successful application in life sciences providing full fingerprint nano-FTIR spectra across intact human cancer cells.

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