Effect of the addition of silicic acid in the medium of callus stimulates the tolerance of pea plants Pisum sativum L. The toxic effects of Rhizoctonia solani

Abstract

Abstract Callus was stimulated from leaves explants of pea seedlings on agar solidified MS medium containing 1.5 mg/L of BA and 2.0 mg/L NAA. Selection of tolerant callus culture virulent strain of Rhizoctonia solani by using the concentration of 12.0 % from callus culture added to it the concentrations of 0.0, 4.0, 8.0, 12.0, 16.0 % of fungal filtrates.Treating callus of using concentrations (10, 102, 103 and 104) µm from salicylic acid over periods (4, 8, 12 and 24) hours followed by callus treated with concentration 12% of filterat fungus for 15 days showed gradual increase in aquiring the callus of brown colour which is compatible to concentrations of salicylic acid in medium and period of treatment. It was also noticed that the best activity of Phenylalanine Ammonia-Lyase and concentration proline was in callus extraction treated with 104µm salicylic acid for 24 hours by using concentration of 12%.

Keywords

• silicic acid
• callus
• pisum sativum l. rhizoctonia solani