CD63-snorkel tagging for isolation of exosomes

Chaoshan Han; Junjie Yang; Tingting Yin; Junqing An; Aijun Qiao; Yangpo Cao; Yuliang Feng; Haocheng Lu; Ying Wang; Liang Yang; Gangjian Qin

Extracellular Vesicle (Dec 2023)

CD63-snorkel tagging for isolation of exosomes

Chaoshan Han,
Junjie Yang,
Tingting Yin,
Junqing An,
Aijun Qiao,
Yangpo Cao,
Yuliang Feng,
Haocheng Lu,
Ying Wang,
Liang Yang,
Gangjian Qin

Affiliations

Chaoshan Han: Department of Pharmacology, School of Medicine, Southern University of Science and Technology, Shenzhen, Guangdong, 518055, PR China
Junjie Yang: Department of Biomedical Engineering, The University of Alabama at Birmingham, School of Medicine and School of Engineering, Birmingham, AL, 35294, USA
Tingting Yin: Department of Pharmacology, School of Medicine, Southern University of Science and Technology, Shenzhen, Guangdong, 518055, PR China
Junqing An: Department of Pharmacology, School of Medicine, Southern University of Science and Technology, Shenzhen, Guangdong, 518055, PR China
Aijun Qiao: Zhongshan Institute for Drug Discovery, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Zhongshan, 528400, PR China; Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai, 201203, PR China
Yangpo Cao: Department of Pharmacology, School of Medicine, Southern University of Science and Technology, Shenzhen, Guangdong, 518055, PR China
Yuliang Feng: Department of Pharmacology, School of Medicine, Southern University of Science and Technology, Shenzhen, Guangdong, 518055, PR China
Haocheng Lu: Department of Pharmacology, School of Medicine, Southern University of Science and Technology, Shenzhen, Guangdong, 518055, PR China
Ying Wang: Department of Pharmacology, School of Medicine, Southern University of Science and Technology, Shenzhen, Guangdong, 518055, PR China
Liang Yang: School of Medicine, Southern University of Science and Technology, Guangdong, 518055, PR China
Gangjian Qin: Department of Pharmacology, School of Medicine, Southern University of Science and Technology, Shenzhen, Guangdong, 518055, PR China; Department of Biomedical Engineering, The University of Alabama at Birmingham, School of Medicine and School of Engineering, Birmingham, AL, 35294, USA; Corresponding author. Department of Pharmacology Cardiovascular Research Institute School of Medicine Southern University of Science and Technology Chuangyuan Building 8, Room 604 1088 Xueyuan Avenue, Nanshan District, Shenzhen, Guangdong, 518055, PR China.

Journal volume & issue: Vol. 2
p. 100031

Abstract

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Exosomes (Exo) are important mediators of inter-cellular communications; however, no effective method is available for isolating, thus characterizing, cellular-specific exosomes in vivo. Since CD63 is a reliable marker for exosomes, we have developed a tagging strategy, term “CD63-Snorkel (CD63-SNKL)”, in which CD63 at its intracellular C-terminus was fused to a fragment of PDGFRB that contains the transmembrane domain tethered to multiple epitope tags (HA, His, and FLAG) displayed in tandem on surface. We found that the CD63-SNKL protein has similar subcellular localizations as endogenous CD63 and can be effectively sorted into Exo. Furthermore, Exo secreted from CD63-SNKL–transduced cells can be effectively captured on anti-HA magnetic beads and eluted with HA peptides. Thus, CD63-SNKL may be engineered for isolating and tracking endogenous tissue-specific Exo in vivo.

Published in Extracellular Vesicle

ISSN: 2773-0417 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Biology (General); Medicine: Medicine (General)
Website: https://www.sciencedirect.com/journal/extracellular-vesicle

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