Bioautomation (Dec 2007)
In vitro-differentiation of Embryonic Mammalian Cells as a Material Base for Gene-engineering Manipulations
Abstract
In in vitro-cultivation and infection of cells from the mammalian cell line EBTr, derived from embryonic bovine trachea, with low initial titers of the DNA vaccine avian pox viral strains FK and Dessau, pathological changes in the inoculated cells were not observed or they were slight. On the other hand, differences between the cells from this line were observed independently of presence or absence of virus infection and they were probably due to their further differentiation. These results indicated that, so applied, the used heterologous for mammals and/or mammalian cells vaccine virus strains, are probably non-pathogenic for them. In agreement with literature data, such safe viral strains might be used for transduction of genes in in vitro- and/or ex vivo-cultivated stem/progenitor cells, by obtained on their bases virus gene constructs.
