Enzymatic oxidation of substituted tryptamines catalysed by monoamine oxidase

Dragulska Sylwia; Kańska Marianna

doi:10.2478/nuka-2014-0015

Nukleonika (Aug 2014)

Enzymatic oxidation of substituted tryptamines catalysed by monoamine oxidase

Dragulska Sylwia,
Kańska Marianna

Affiliations

Dragulska Sylwia: Department of Chemistry, University of Warsaw, 1 Pasteura Str., 02-093 Warsaw, Poland, Tel.: +48 22 822 0211 ext. 509, Fax: +48 22 822 0211 ext. 434
Kańska Marianna: Department of Chemistry, University of Warsaw, 1 Pasteura Str., 02-093 Warsaw, Poland, Tel.: +48 22 822 0211 ext. 509, Fax: +48 22 822 0211 ext. 434

DOI: https://doi.org/10.2478/nuka-2014-0015
Journal volume & issue: Vol. 59, no. 3
pp. 91 – 95

Abstract

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The enzymatic deamination of 5-fl uorotryptamine and 5-hydroxytryptamine, 5-HT, catalysed by enzyme monoamine oxidase A (MAO-A, EC 1.4.3.4) was investigated using the kinetic (KIE) and solvent (SIE) isotope effects methods. The numerical values of deuterium isotope effects in the (1R) positions of 5-F-tryptamine were determined using non-competitive spectrophotomeric method. Isotopologue 5-F-[(1R)- -2H]-tryptamine, needed for kinetic studies was obtained by enzymatic decarboxylation of 5´-fl uoro-L-tryptophan, 5´-F-L-Trp, in fully deuteriated medium.

Published in Nukleonika

ISSN: 0029-5922 (Print); 1508-5791 (Online)
Publisher: Sciendo
Country of publisher: Poland
LCC subjects: Science
Website: https://sciendo.com/journal/NUKA

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