A comparative analysis of beta-cypermethrin nanoemulsion and traditional emulsion dosages on enzyme activities in Blattella germanica
Yan Shen,
Qiong Li,
Fujin Fang,
Chuanli Yang,
Yu Dong,
Xiaoqin Li,
Zhizhi Luo,
Xiaobing Shen
Affiliations
Yan Shen
Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, 210009, Jiangsu, China; School of Public Health, Southeast University, Nanjing, 210009, Jiangsu, China
Qiong Li
Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, 210009, Jiangsu, China; School of Public Health, Southeast University, Nanjing, 210009, Jiangsu, China
Fujin Fang
Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, 210009, Jiangsu, China; School of Public Health, Southeast University, Nanjing, 210009, Jiangsu, China
Chuanli Yang
Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, 210009, Jiangsu, China; School of Public Health, Southeast University, Nanjing, 210009, Jiangsu, China
Yu Dong
Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, 210009, Jiangsu, China; School of Public Health, Southeast University, Nanjing, 210009, Jiangsu, China
Xiaoqin Li
Yangzhou Disease Control and Prevention Center, Yangzhou, 225007, China
Zhizhi Luo
Yangzhou Disease Control and Prevention Center, Yangzhou, 225007, China
Xiaobing Shen
Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, 210009, Jiangsu, China; School of Public Health, Southeast University, Nanjing, 210009, Jiangsu, China; Corresponding author. Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, 210009, Jiangsu, China.
Objective: To compare the impact of two pesticide dose forms on Blattella germanica (B. germanica)resistance enzymes.The micro-drop technique was utilized on subcultured B. germanica, and the metabolic enzyme activity was assessed.Using spectrophotometry, the relative enzyme activities of B.germanica were determined.Profile analysis was used to compare the enzyme activities of beta-cypermethrin in both nanoemulsion and traditional emulsion forms.Findings: The suppression percentages of the acetylcholinesterase enzyme (AchE), GST, and P450–O demethylases across different pesticide formulations were analyzed using regression equations, yielding F = 31.18, P < 0.001; F = 9.18, P < 0.001; and F = 4.58, P < 0.02.Conclusion: The suppression of AchE, GST, and P450–O demethylase by beta-cypermethrin nanoemulsion was more significant compared to the beta-cypermethrin emulsifiable concentrate.The study concluded that beta-cypermethrin nanoemulsion had a significant impact on insect detoxifying enzymes compared to beta-cypermethrin emulsifiable concentrate due to their numerous advantages.
