New Application of the Commercially Available Dye Celestine Blue B as a Sensitive and Selective Fluorescent “Turn-On” Probe for Endogenous Detection of HOCl and Reactive Halogenated Species
Veronika E. Reut,
Stanislav O. Kozlov,
Igor V. Kudryavtsev,
Natalya A. Grudinina,
Valeria A. Kostevich,
Nikolay P. Gorbunov,
Daria V. Grigorieva,
Julia A. Kalvinkovskaya,
Sergey B. Bushuk,
Elena Yu Varfolomeeva,
Natalia D. Fedorova,
Irina V. Gorudko,
Oleg M. Panasenko,
Vadim B. Vasilyev,
Alexey V. Sokolov
Affiliations
Veronika E. Reut
Department of Biophysics, Belarusian State University, 220030 Minsk, Belarus
Stanislav O. Kozlov
Department of Molecular Genetics, Institute of Experimental Medicine, 197376 Saint-Petersburg, Russia
Igor V. Kudryavtsev
Department of Immunology, Institute of Experimental Medicine, 197376 Saint-Petersburg, Russia
Natalya A. Grudinina
Department of Molecular Genetics, Institute of Experimental Medicine, 197376 Saint-Petersburg, Russia
Valeria A. Kostevich
Department of Molecular Genetics, Institute of Experimental Medicine, 197376 Saint-Petersburg, Russia
Nikolay P. Gorbunov
Department of Molecular Genetics, Institute of Experimental Medicine, 197376 Saint-Petersburg, Russia
Daria V. Grigorieva
Department of Biophysics, Belarusian State University, 220030 Minsk, Belarus
Julia A. Kalvinkovskaya
Stepanov Institute of Physics, National Academy of Sciences of Belarus, 220072 Minsk, Belarus
Sergey B. Bushuk
SSPA “Optics, Optoelectronics, and Laser Technology”, 220072 Minsk, Belarus
Elena Yu Varfolomeeva
Petersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre “Kurchatov Institute”, 188300 Gatchina, Russia
Natalia D. Fedorova
Petersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre “Kurchatov Institute”, 188300 Gatchina, Russia
Irina V. Gorudko
Department of Biophysics, Belarusian State University, 220030 Minsk, Belarus
Oleg M. Panasenko
Department of Biophysics, Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, Russia
Vadim B. Vasilyev
Department of Molecular Genetics, Institute of Experimental Medicine, 197376 Saint-Petersburg, Russia
Alexey V. Sokolov
Department of Molecular Genetics, Institute of Experimental Medicine, 197376 Saint-Petersburg, Russia
Hypochlorous acid (HOCl) derived from hydrogen peroxide and chloride anion by myeloperoxidase (MPO) plays a significant role in physiological and pathological processes. Herein we report a phenoxazine-based fluorescent probe Celestine Blue B (CB) that is applicable for HOCl detection in living cells and for assaying the chlorinating activity of MPO. A remarkable selectivity and sensitivity (limit of detection is 32 nM), along with a rapid “turn-on” response of CB to HOCl was demonstrated. Furthermore, the probe was able to detect endogenous HOCl and reactive halogenated species by fluorescence spectroscopy, confocal microscopy, and flow cytometry techniques. Hence, CB is a promising tool for investigating the role of HOCl in health and disease and for screening the drugs capable of regulating MPO activity.
