Thiolated Chitosan Conjugated Liposomes for Oral Delivery of Selenium Nanoparticles
Atiđa Selmani,
Elisabeth Seibert,
Carolin Tetyczka,
Doris Kuehnelt,
Ivan Vidakovic,
Karin Kornmueller,
Markus Absenger-Novak,
Borna Radatović,
Ivana Vinković Vrček,
Gerd Leitinger,
Eleonore Fröhlich,
Andreas Bernkop-Schnürch,
Eva Roblegg,
Ruth Prassl
Affiliations
Atiđa Selmani
Department of Pharmaceutical Technology and Biopharmacy, Institute of Pharmaceutical Sciences, University of Graz, 8010 Graz, Austria
Elisabeth Seibert
Division of Biophysics, Gottfried Schatz Research Center for Cell Signaling, Metabolism and Aging, Medical University of Graz, 8010 Graz, Austria
Carolin Tetyczka
Department of Pharmaceutical Technology and Biopharmacy, Institute of Pharmaceutical Sciences, University of Graz, 8010 Graz, Austria
Doris Kuehnelt
Institute of Chemistry, Analytical Chemistry, NAWI Graz, University of Graz, 8010 Graz, Austria
Ivan Vidakovic
Division of Biophysics, Gottfried Schatz Research Center for Cell Signaling, Metabolism and Aging, Medical University of Graz, 8010 Graz, Austria
Karin Kornmueller
Division of Biophysics, Gottfried Schatz Research Center for Cell Signaling, Metabolism and Aging, Medical University of Graz, 8010 Graz, Austria
Markus Absenger-Novak
Center for Medical Research, Medical University of Graz, 8010 Graz, Austria
Borna Radatović
Center of Excellence for Advanced Materials and Sensing Devices, Institute of Physics, 10000 Zagreb, Croatia
Ivana Vinković Vrček
Institute for Medical Research and Occupational Health, 10000 Zagreb, Croatia
Gerd Leitinger
Division of Cell Biology, Histology and Embryology, Gottfried Schatz Research Center for Cell Signaling, Metabolism and Aging, Medical University of Graz, 8010 Graz, Austria
Eleonore Fröhlich
Center for Medical Research, Medical University of Graz, 8010 Graz, Austria
Andreas Bernkop-Schnürch
Department of Pharmaceutical Technology, Center for Chemistry and Biomedicine, Institute of Pharmacy, University of Innsbruck, 6020 Innsbruck, Austria
Eva Roblegg
Department of Pharmaceutical Technology and Biopharmacy, Institute of Pharmaceutical Sciences, University of Graz, 8010 Graz, Austria
Ruth Prassl
Division of Biophysics, Gottfried Schatz Research Center for Cell Signaling, Metabolism and Aging, Medical University of Graz, 8010 Graz, Austria
This study aimed to design a hybrid oral liposomal delivery system for selenium nanoparticles (Lip-SeNPs) to improve the bioavailability of selenium. Thiolated chitosan, a multifunctional polymer with mucoadhesive properties, was used for surface functionalization of Lip-SeNPs. Selenium nanoparticle (SeNP)-loaded liposomes were manufactured by a single step microfluidics-assisted chemical reduction and assembling process. Subsequently, chitosan-N-acetylcysteine was covalently conjugated to the preformed Lip-SeNPs. The Lip-SeNPs were characterized in terms of composition, morphology, size, zeta potential, lipid organization, loading efficiency and radical scavenging activity. A co-culture system (Caco-2:HT29-MTX) that integrates mucus secreting and enterocyte-like cell types was used as a model of the human intestinal epithelium to determine adsorption, mucus penetration, release and transport properties of Lip-SeNPs in vitro. Thiolated Lip-SeNPs were positively charged with an average size of about 250 nm. Thiolated Lip-SeNPs tightly adhered to the mucus layer without penetrating the enterocytes. This finding was consistent with ex vivo adsorption studies using freshly excised porcine small intestinal tissues. Due to the improved mucoadhesion and retention in a simulated microenvironment of the small intestine, thiolated Lip-SeNPs might be a promising tool for oral selenium delivery.
