Fast, Sensitive and Specific Detection of <i>Thailand orthohantavirus</i> and Its Variants Using One-Step Real-Time Reverse-Transcription Polymerase Chain Reaction Assay
Vololoniaina Raharinosy,
Jean-Michel Heraud,
Harinirina Aina Rabemananjara,
Sandra Telfer,
Danielle Aurore Doll Rakoto,
Claudia Filippone,
Jean-Marc Reynes
Affiliations
Vololoniaina Raharinosy
Virology Unit, Institut Pasteur de Madagascar, 101 Antananarivo, Madagascar
Jean-Michel Heraud
Virology Unit, Institut Pasteur de Madagascar, 101 Antananarivo, Madagascar
Harinirina Aina Rabemananjara
Virology Unit, Institut Pasteur de Madagascar, 101 Antananarivo, Madagascar
Sandra Telfer
Plague Unit, Institut Pasteur de Madagascar, 101 Antananarivo, Madagascar
Danielle Aurore Doll Rakoto
Ecole Doctorale Science de la Vie et de l’Environnement, Faculté des Sciences, Université d’Antananarivo, 101 Antananarivo, Madagascar
Claudia Filippone
Virology Unit, Institut Pasteur de Madagascar, 101 Antananarivo, Madagascar
Jean-Marc Reynes
Unité de Biologie des Infections Virales Emergentes, Institut Pasteur, 69000 Lyon, France
Genetic variants of Thailand orthohantavirus (THAIV) have been recently reported from rodents in South-East Asia and in islands from the South-West part of the Indian Ocean. In order to detect THAIV and its variants, we developed a sensitive and specific real-time RT-PCR targeting the S segment. Our assay was developed in two different RT-PCR systems that gave similar results in terms of sensitivity. Moreover, our results demonstrated a specificity of 100%.
