Flexible Multiplexed In2O3 Nanoribbon Aptamer-Field-Effect Transistors for Biosensing
Qingzhou Liu,
Chuanzhen Zhao,
Mingrui Chen,
Yihang Liu,
Zhiyuan Zhao,
Fanqi Wu,
Zhen Li,
Paul S. Weiss,
Anne M. Andrews,
Chongwu Zhou
Affiliations
Qingzhou Liu
Ming Hsieh Department of Electrical Engineering, University of Southern California, Los Angeles, CA 90089, USA; Mork Family Department of Chemical Engineering and Materials Science, University of Southern California, Los Angeles, CA 90089, USA
Chuanzhen Zhao
Department of Chemistry and Biochemistry, California NanoSystems Institute, University of California, Los Angeles, Los Angeles, CA 90095, USA
Mingrui Chen
Ming Hsieh Department of Electrical Engineering, University of Southern California, Los Angeles, CA 90089, USA
Yihang Liu
Mork Family Department of Chemical Engineering and Materials Science, University of Southern California, Los Angeles, CA 90089, USA
Zhiyuan Zhao
Ming Hsieh Department of Electrical Engineering, University of Southern California, Los Angeles, CA 90089, USA
Fanqi Wu
Ming Hsieh Department of Electrical Engineering, University of Southern California, Los Angeles, CA 90089, USA
Zhen Li
Mork Family Department of Chemical Engineering and Materials Science, University of Southern California, Los Angeles, CA 90089, USA
Paul S. Weiss
Department of Chemistry and Biochemistry, California NanoSystems Institute, University of California, Los Angeles, Los Angeles, CA 90095, USA; Departments of Bioengineering and Materials Science and Engineering, University of California, Los Angeles, Los Angeles, CA 90095, USA; Corresponding author
Anne M. Andrews
Department of Chemistry and Biochemistry, California NanoSystems Institute, University of California, Los Angeles, Los Angeles, CA 90095, USA; Department of Psychiatry and Biobehavioral Sciences, Semel Institute for Neuroscience and Human Behavior, and Hatos Center for Neuropharmacology, University of California, Los Angeles, Los Angeles, CA 90095, USA; Corresponding author
Chongwu Zhou
Ming Hsieh Department of Electrical Engineering, University of Southern California, Los Angeles, CA 90089, USA; Mork Family Department of Chemical Engineering and Materials Science, University of Southern California, Los Angeles, CA 90089, USA; Corresponding author
Summary: Flexible sensors are essential for advancing implantable and wearable bioelectronics toward monitoring chemical signals within and on the body. Developing biosensors for monitoring multiple neurotransmitters in real time represents a key in vivo application that will increase understanding of information encoded in brain neurochemical fluxes. Here, arrays of devices having multiple In2O3 nanoribbon field-effect transistors (FETs) were fabricated on 1.4-μm-thick polyethylene terephthalate (PET) substrates using shadow mask patterning techniques. Thin PET-FET devices withstood crumpling and bending such that stable transistor performance with high mobility was maintained over >100 bending cycles. Real-time detection of the small-molecule neurotransmitters serotonin and dopamine was achieved by immobilizing recently identified high-affinity nucleic-acid aptamers on individual In2O3 nanoribbon devices. Limits of detection were 10 fM for serotonin and dopamine with detection ranges spanning eight orders of magnitude. Simultaneous sensing of temperature, pH, serotonin, and dopamine enabled integration of physiological and neurochemical data from individual bioelectronic devices.
