A New Type of LSPR Sensor Featuring Immobilized Liposome or Phospholipid Single Layer

Abstract

We have fabricated a new type of LSPR sensor featuring immobilized liposome or phospholipid single layer. LSPR principally shows an ultrahigh sensitivity on surface dielectric environmental change due to interaction with target, but little has been reported so far on applying phospholipid membranes and/or liposomes as model cell membrane. We newly tried to investigate biosensing capabilities using the membranes of the both structures on Au nanostructures of LSPR sensor chip. As a result, it was confirmed that the phospholipid single layer is more effective to improve the sensitivity than the liposome. Finally, we have clearly detected 100 nM target protein of CAB and estimated a possible detection of 10 nM range from wavelength resolution by interaction with the phospholipid single layer.

Keywords

• LSPR
• biosensor
• nanostructure
• nanopillar
• phospholipid
• liposome
• high-sensitive detection