Genome-Wide Identification of the <i>TIFY</i> Gene Family in <i>Brassiceae</i> and Its Potential Association with Heavy Metal Stress in Rapeseed
Fujun Sun,
Zhiyou Chen,
Qianwei Zhang,
Yuanyuan Wan,
Ran Hu,
Shulin Shen,
Si Chen,
Nengwen Yin,
Yunshan Tang,
Ying Liang,
Kun Lu,
Cunmin Qu,
Wei Hua,
Jiana Li
Affiliations
Fujun Sun
Chongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology and Academy of Agricultural Sciences, Southwest University, Chongqing 400716, China
Zhiyou Chen
Chongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology and Academy of Agricultural Sciences, Southwest University, Chongqing 400716, China
Qianwei Zhang
Chongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology and Academy of Agricultural Sciences, Southwest University, Chongqing 400716, China
Yuanyuan Wan
Chongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology and Academy of Agricultural Sciences, Southwest University, Chongqing 400716, China
Ran Hu
Chongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology and Academy of Agricultural Sciences, Southwest University, Chongqing 400716, China
Shulin Shen
Chongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology and Academy of Agricultural Sciences, Southwest University, Chongqing 400716, China
Si Chen
Chongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology and Academy of Agricultural Sciences, Southwest University, Chongqing 400716, China
Nengwen Yin
Chongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology and Academy of Agricultural Sciences, Southwest University, Chongqing 400716, China
Yunshan Tang
Chongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology and Academy of Agricultural Sciences, Southwest University, Chongqing 400716, China
Ying Liang
Chongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology and Academy of Agricultural Sciences, Southwest University, Chongqing 400716, China
Kun Lu
Chongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology and Academy of Agricultural Sciences, Southwest University, Chongqing 400716, China
Cunmin Qu
Chongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology and Academy of Agricultural Sciences, Southwest University, Chongqing 400716, China
Wei Hua
Key Laboratory of Biology and Genetic Improvement of Oil Crops, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Ministry of Agriculture and Rural Affairs, Wuhan 430062, China
Jiana Li
Chongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology and Academy of Agricultural Sciences, Southwest University, Chongqing 400716, China
The TIFY gene family plays important roles in various plant biological processes and responses to stress and hormones. The chromosome-level genome of the Brassiceae species has been released, but knowledge concerning the TIFY family is lacking in the Brassiceae species. The current study performed a bioinformatics analysis on the TIFY family comparing three diploid (B. rapa, B. nigra, and B. oleracea) and two derived allotetraploid species (B. juncea, and B. napus). A total of 237 putative TIFY proteins were identified from five Brassiceae species, and classified into ten subfamilies (six JAZ types, one PPD type, two TIFY types, and one ZML type) based on their phylogenetic relationships with TIFY proteins in A. thaliana and Brassiceae species. Duplication and synteny analysis revealed that segmental and tandem duplications led to the expansion of the TIFY family genes during the process of polyploidization, and most of these TIFY family genes (TIFYs) were subjected to purifying selection after duplication based on Ka/Ks values. The spatial and temporal expression patterns indicated that different groups of BnaTIFYs have distinct spatiotemporal expression patterns under normal conditions and heavy metal stresses. Most of the JAZIII subfamily members were highest in all tissues, but JAZ subfamily members were strongly induced by heavy metal stresses. BnaTIFY34, BnaTIFY59, BnaTIFY21 and BnaTIFY68 were significantly upregulated mostly under As3+ and Cd2+ treatment, indicating that they could be actively induced by heavy metal stress. Our results may contribute to further exploration of TIFYs, and provided valuable information for further studies of TIFYs in plant tolerance to heavy metal stress.
