Frontiers in Cell and Developmental Biology (Nov 2021)

The m6A RNA Modification Modulates Gene Expression and Fibrosis-Related Pathways in Hypertrophic Scar

  • Si-Yu Liu,
  • Jun-Jie Wu,
  • Zhong-hua Chen,
  • Ming-Li Zou,
  • Ying-ying Teng,
  • Kai-Wen Zhang,
  • Yue-Yue Li,
  • Dang-yang Guo,
  • Feng-Lai Yuan,
  • Feng-Lai Yuan,
  • Feng-Lai Yuan

DOI
https://doi.org/10.3389/fcell.2021.748703
Journal volume & issue
Vol. 9

Abstract

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Purpose: To systematically analyze the overall m6A modification pattern in hyperplastic scars (HS).Methods: The m6A modification patterns in HS and normal skin (NS) tissues were described by m6A sequencing and RNA sequencing, and subsequently bioinformatics analysis was performed. The m6A-related RNA was immunoprecipitated and verified by real-time quantitative PCR.Results: The appearance of 14,791 new m6A peaks in the HS sample was accompanied by the disappearance of 7,835 peaks. The unique m6A-related genes in HS were thus associated with fibrosis-related pathways. We identified the differentially expressed mRNA transcripts in HS samples with hyper-methylated or hypo-methylated m6A peaks.Conclusion: This study is the first to map the m6A transcriptome of human HS, which may help clarify the possible mechanism of m6A-mediated gene expression regulation.

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