PLoS ONE (Jan 2020)

High sPLA2-IIA level is associated with eicosanoid metabolism in patients with bacterial sepsis syndrome.

  • Nurul Saadah Ahmad,
  • Toh Leong Tan,
  • Khaizurin Tajul Arifin,
  • Wan Zurinah Wan Ngah,
  • Yasmin Anum Mohd Yusof

DOI
https://doi.org/10.1371/journal.pone.0230285
Journal volume & issue
Vol. 15, no. 3
p. e0230285

Abstract

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The aim of this study was to determine the association between secretory phospholipase A2 group IIA (sPLA2-IIA) and eicosanoid pathway metabolites in patients with bacterial sepsis syndrome (BSS). Levels of sPLA2-IIA, eicosanoids prostaglandin (PG)E2, PGD synthase were quantified in the sera from patients confirmed to have bacterial sepsis (BS; N = 45), bacterial severe sepsis/septic shock (BSS/SS; N = 35) and healthy subjects (N = 45). Cyclooxygenase (COX)-1 and COX-2 activities were analyzed from cell lysate. Serum levels of sPLA2-IIA, PGE2, and PGDS increased significantly in patients with BS and BSS/SS compared to healthy subjects (p<0.05). COX-2 activity was significantly increased in patients with BS compared to healthy subjects (p<0.05), but not COX-1 activity. Binary logistic regression analysis showed that sPLA2-IIA and PGE2 were independent factors predicting BSS severity. In conclusion, high level of sPLA2-IIA is associated with eicosanoid metabolism in patients with BSS.