SARS-CoV-2 Multi-Antigen Serology Assay

Ramin Mazhari; Shazia Ruybal-Pesántez; Fiona Angrisano; Nicholas Kiernan-Walker; Stephanie Hyslop; Rhea J. Longley; Caitlin Bourke; Catherine Chen; Deborah A. Williamson; Leanne J. Robinson; Ivo Mueller; Emily M. Eriksson

doi:10.3390/mps4040072

Methods and Protocols (Oct 2021)

SARS-CoV-2 Multi-Antigen Serology Assay

Ramin Mazhari,
Shazia Ruybal-Pesántez,
Fiona Angrisano,
Nicholas Kiernan-Walker,
Stephanie Hyslop,
Rhea J. Longley,
Caitlin Bourke,
Catherine Chen,
Deborah A. Williamson,
Leanne J. Robinson,
Ivo Mueller,
Emily M. Eriksson

Affiliations

Ramin Mazhari: Walter and Eliza Hall Institute of Medical Research, Melbourne, VIC 3052, Australia
Shazia Ruybal-Pesántez: Walter and Eliza Hall Institute of Medical Research, Melbourne, VIC 3052, Australia
Fiona Angrisano: Vector-Borne Diseases and Tropical Public Health, Burnet Institute, Melbourne, VIC 3004, Australia
Nicholas Kiernan-Walker: Walter and Eliza Hall Institute of Medical Research, Melbourne, VIC 3052, Australia
Stephanie Hyslop: Walter and Eliza Hall Institute of Medical Research, Melbourne, VIC 3052, Australia
Rhea J. Longley: Walter and Eliza Hall Institute of Medical Research, Melbourne, VIC 3052, Australia
Caitlin Bourke: Walter and Eliza Hall Institute of Medical Research, Melbourne, VIC 3052, Australia
Catherine Chen: Walter and Eliza Hall Institute of Medical Research, Melbourne, VIC 3052, Australia
Deborah A. Williamson: Royal Melbourne Hospital, Melbourne, VIC 3052, Australia
Leanne J. Robinson: Walter and Eliza Hall Institute of Medical Research, Melbourne, VIC 3052, Australia
Ivo Mueller: Walter and Eliza Hall Institute of Medical Research, Melbourne, VIC 3052, Australia
Emily M. Eriksson: Walter and Eliza Hall Institute of Medical Research, Melbourne, VIC 3052, Australia

DOI: https://doi.org/10.3390/mps4040072
Journal volume & issue: Vol. 4, no. 4
p. 72

Abstract

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Serology tests are extremely useful for assessing whether a person has been infected with a pathogen. Since the onset of the COVID-19 pandemic, measurement of anti-SARS-CoV-2-specific antibodies has been considered an essential tool in identifying seropositive individuals and thereby understanding the extent of transmission in communities. The Luminex system is a bead-based technology that has the capacity to assess multiple antigens simultaneously using very low sample volumes and is ideal for high-throughput studies. We have adapted this technology to develop a COVID-19 multi-antigen serological assay. This protocol described here carefully outlines recommended steps to optimize and establish this method for COVID-19-specific antibody measurement in plasma and in saliva. However, the protocol can easily be customized and thus the assay is broadly applicable to measure antibodies to other pathogens.

Published in Methods and Protocols

ISSN: 2409-9279 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Biology (General)
Website: https://www.mdpi.com/journal/mps

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