sncRNAs packaged by Helicobacter pylori outer membrane vesicles attenuate IL-8 secretion in human cells

Hongxia Zhang; Yingxuan Zhang; Zifan Song; Ruizhen Li; Huan Ruan; Qiong Liu; Xiaotian Huang

International Journal of Medical Microbiology (Jan 2020)

sncRNAs packaged by Helicobacter pylori outer membrane vesicles attenuate IL-8 secretion in human cells

Hongxia Zhang,
Yingxuan Zhang,
Zifan Song,
Ruizhen Li,
Huan Ruan,
Qiong Liu,
Xiaotian Huang

Affiliations

Hongxia Zhang: Department of Medical Microbiology, School of Medicine, Nanchang University, Nanchang, 330006, China
Yingxuan Zhang: The First Clinical Medical College, Nanchang University, Nanchang, 330006, China
Zifan Song: The First Clinical Medical College, Nanchang University, Nanchang, 330006, China
Ruizhen Li: The First Clinical Medical College, Nanchang University, Nanchang, 330006, China
Huan Ruan: Department of Medical Microbiology, School of Medicine, Nanchang University, Nanchang, 330006, China
Qiong Liu: Department of Medical Microbiology, School of Medicine, Nanchang University, Nanchang, 330006, China; Corresponding authors at: Department of Medical Microbiology, School of Medicine, Nanchang University, Nanchang, 330006, China.
Xiaotian Huang: Department of Medical Microbiology, School of Medicine, Nanchang University, Nanchang, 330006, China; Key Laboratory of Tumor Pathogenesis and Molecular Pathology, School of Medicine, Nanchang University, Nanchang, 330006, China; Corresponding authors at: Department of Medical Microbiology, School of Medicine, Nanchang University, Nanchang, 330006, China.

Journal volume & issue: Vol. 310, no. 1

Abstract

Read online

Bacterial outer membrane vesicles (OMVs) play a vital role in the mechanism of host―pathogen communication, while emerging evidence suggests that OMVs regulate host immune responses through differentially packaged small noncoding RNAs (sncRNAs) to target host mRNA function. Therefore, we identified differentially packaged sncRNAs in Helicobacter pylori OMVs and showed transfer of OMV sncRNAs to human gastric adenocarcinoma cells in this study. Our data revealed that sncRNAs (sR-2509025 and sR-989262) were enriched in OMVs, and reduced lipopolysaccharide or OMV-induced interleukin 8 (IL-8) secretion by cultured AGS cells. Collectively, these findings are consistent with the hypothesis that sncRNAs in H. pylori OMVs play a novel role in the mechanism of host―pathogen interaction, whereby H. pylori evades the host immune response.

Published in International Journal of Medical Microbiology

ISSN: 1438-4221 (Print); 1618-0607 (Online)
Publisher: Elsevier
Country of publisher: Germany
LCC subjects: Science: Microbiology; Medicine: Other systems of medicine
Website: https://www.journals.elsevier.com/international-journal-of-medical-microbiology

About the journal

Abstract

Keywords