Acta Cirúrgica Brasileira (Apr 2017)

Development of a new model of actinic enteritis in rats using a cobalt-60 open source and a protection device as a collimator

  • Vinicius Rodrigues Taranto Nunes,
  • Paula Vieira Teixeira Vidigal,
  • Márcio Tadeu Pereira,
  • Luís Carlos Duarte Ladeira,
  • Rafael Calvão Barbuto,
  • Ivana Duval-Araujo

DOI
https://doi.org/10.1590/s0102-865020170040000007
Journal volume & issue
Vol. 32, no. 4
pp. 319 – 324

Abstract

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Abstract Purpose: To describe a new model of actinic enteritis that does not use radiotherapy machines. Methods: Sixteen Wistar rats were divided into four groups, consisting of four animals each: control (group A), two weeks after irradiation (group B), five weeks after irradiation (group C) and eight weeks after irradiation (group D). Animals were given a 10Gy radiation from a Cobalt-60 natural source in a nuclear technology research center. Protections of the surrounding tissues were obtained through the usage of plumb devices with a hole in the center, which served as a collimator. We obtained irradiated and non-irradiated colons from each animal. Results: In group B we found an important inflammatory response in the irradiated colon, which appeared in a reduced way in group C and was minimal in group D, in which we found a relevant collagen submucosal deposition/fibrosis. In all groups, the non-irradiated colon had a lower pathological damage in comparison with the irradiated colon. Conclusion: We thus described an efficient and feasible technique for obtaining an animal model of actinic enteritis.

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