Eutypellenoids A–C, New Pimarane Diterpenes from the Arctic Fungus Eutypella sp. D-1
Hao-Bing Yu,
Xiao-Li Wang,
Wei-Heng Xu,
Yi-Xin Zhang,
Yi-Sen Qian,
Jian-Peng Zhang,
Xiao-Ling Lu,
Xiao-Yu Liu
Affiliations
Hao-Bing Yu
Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, and Marine Biopharmaceutical Institute, Second Military Medical University, Shanghai 200433, China
Xiao-Li Wang
Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, and Marine Biopharmaceutical Institute, Second Military Medical University, Shanghai 200433, China
Wei-Heng Xu
School of Pharmacy, Second Military Medical University, Shanghai 200433, China
Yi-Xin Zhang
Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, and Marine Biopharmaceutical Institute, Second Military Medical University, Shanghai 200433, China
Yi-Sen Qian
School of Physical Sciences, University of California, Irvine, CA 92697, USA
Jian-Peng Zhang
Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, and Marine Biopharmaceutical Institute, Second Military Medical University, Shanghai 200433, China
Xiao-Ling Lu
Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, and Marine Biopharmaceutical Institute, Second Military Medical University, Shanghai 200433, China
Xiao-Yu Liu
Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, and Marine Biopharmaceutical Institute, Second Military Medical University, Shanghai 200433, China
Three new pimarane diterpenes, eutypellenoids A–C (1–3), together with a known compound, eutypenoid C (4), were isolated from the culture extract of Eutypella sp. D-1 derived from the Arctic region. Compounds 1–3 possessed an uncommon tetrahydrofuran-fused pimarane diterpene skeleton. The structures of all compounds were determined by detailed spectroscopic analysis, electronic circular dichroism (ECD) analysis, as well as a comparison with the literature data. Antibacterial, antifungal, and cytotoxic activities of these compounds were evaluated. Compound 2 displayed antibacterial activity against Staphylococcus aureus and Escherichia coli with MIC values of 8 and 8 μg/mL, respectively. Additionally, compound 2 showed antifungal activity against Candida parapsilosis, Candida albicans, Candida glabrata, and Candida tropicalis with MIC values of 8, 8, 16, and 32 μg/mL, respectively. Furthermore, compound 2 exhibited moderate cytotoxic activity against HCT-116 cell line with IC50 value of 3.7 μM.
