Knockout of Dip2c in murine ES cell line IBMSe001-B-1 by CRISPR/Cas9 genome editing technology

Mingze Yao; Pengfei Su; Zhengfeng Li; Xiaogang Cui; Qian Yang; Xiaoyu Xing; Yu Lei; Mengqiao Lian; Tinglin Ren; Xiedong Wang; Yaowu Zheng; Changxin Wu

Stem Cell Research (May 2021)

Knockout of Dip2c in murine ES cell line IBMSe001-B-1 by CRISPR/Cas9 genome editing technology

Mingze Yao,
Pengfei Su,
Zhengfeng Li,
Xiaogang Cui,
Qian Yang,
Xiaoyu Xing,
Yu Lei,
Mengqiao Lian,
Tinglin Ren,
Xiedong Wang,
Yaowu Zheng,
Changxin Wu

Affiliations

Mingze Yao: Institutes of Biomedical Sciences, Shanxi University, No. 92 Wucheng Road, 030006 Taiyuan, China; Corresponding authors at: Institutes of Biomedical Sciences, Shanxi University, No. 92 Wucheng Road, 030006 Taiyuan, China.
Pengfei Su: Institutes of Biomedical Sciences, Shanxi University, No. 92 Wucheng Road, 030006 Taiyuan, China
Zhengfeng Li: Faculty of Health Sciences, University of Macau, Avenida da Universidade, Taipa, Macau, China
Xiaogang Cui: Institutes of Biomedical Sciences, Shanxi University, No. 92 Wucheng Road, 030006 Taiyuan, China
Qian Yang: Institutes of Biomedical Sciences, Shanxi University, No. 92 Wucheng Road, 030006 Taiyuan, China
Xiaoyu Xing: Institutes of Biomedical Sciences, Shanxi University, No. 92 Wucheng Road, 030006 Taiyuan, China
Yu Lei: Institutes of Biomedical Sciences, Shanxi University, No. 92 Wucheng Road, 030006 Taiyuan, China
Mengqiao Lian: Institutes of Biomedical Sciences, Shanxi University, No. 92 Wucheng Road, 030006 Taiyuan, China
Tinglin Ren: Institutes of Biomedical Sciences, Shanxi University, No. 92 Wucheng Road, 030006 Taiyuan, China
Xiedong Wang: Institutes of Biomedical Sciences, Shanxi University, No. 92 Wucheng Road, 030006 Taiyuan, China
Yaowu Zheng: Institutes of Biomedical Sciences, Shanxi University, No. 92 Wucheng Road, 030006 Taiyuan, China; Transgenic Research Center, Northeast Normal University, Changchun, China; Corresponding authors at: Institutes of Biomedical Sciences, Shanxi University, No. 92 Wucheng Road, 030006 Taiyuan, China.
Changxin Wu: Institutes of Biomedical Sciences, Shanxi University, No. 92 Wucheng Road, 030006 Taiyuan, China; Corresponding authors at: Institutes of Biomedical Sciences, Shanxi University, No. 92 Wucheng Road, 030006 Taiyuan, China.

Journal volume & issue: Vol. 53
p. 102236

Abstract

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DIP2 protein contains three members: DIP2A, DIP2B and DIP2C, and are broadly expressed in the nervous system from Drosophila to human during embryonic development. Dip2c gene-associated mutations have been reported in tumors and neuronal diseases. However, the role of Dip2c in the context of mouse embryonic stem (mES) cells has not been explored. To investigate the biological function of Dip2c during early embryo development, we generated Dip2c-/- mES line using a CRISPR/Cas9 system. This cell line has contributed to further investigation of molecular mechanism of Dip2c during cell differentiation, as well as a cell model for screening for neurogenic drug and cancer clinical cure.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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