The second complete chloroplast genome sequence of Nymphaea alba L. (Nymphaeaceae) to investigate inner-species variations

Abstract

Nymphaea alba L. which belongs to Nymphaeaceae has been used as gardening plants, foods, and medicines. In this study, we completed whole chloroplast genome of N. alba of which length is 151,925 bp consisting four subregions: 90,001 bp of large single copy (LSC) and 19,562 bp of small single copy (SSC) regions are separated by 25,181 bp of inverted repeat (IR) regions. One hundred and twenty-eight genes (84 protein-coding genes, 8 rRNAs, and 36 tRNAs) were annotated successfully. The overall GC content of the chloroplast genome is 39.2% and those in the LSC, SSC, and IR regions are 37.8%, 34.4%, and 43.4%, respectively. Eleven single nucleotide polymorphisms and six insertion and deletions are identified between two N. alba chloroplast genomes. This genome will be used for developing molecular markers and for understating micro-evolutionary events of this species.

Keywords

• nymphaea
• chloroplast genome
• nymphaea alba
• nymphaceae
• inner-species variation