Analyzing the Influence of Imaging Resolution on Polarization Properties of Scattering Media Obtained From Mueller Matrix

Conghui Shao; Conghui Shao; Binguo Chen; Binguo Chen; Binguo Chen; Honghui He; Honghui He; Chao He; Yuanxing Shen; Yuanxing Shen; Yuanxing Shen; Haoyu Zhai; Haoyu Zhai; Haoyu Zhai; Hui Ma; Hui Ma; Hui Ma

doi:10.3389/fchem.2022.936255

Frontiers in Chemistry (Jul 2022)

Analyzing the Influence of Imaging Resolution on Polarization Properties of Scattering Media Obtained From Mueller Matrix

Conghui Shao,
Conghui Shao,
Binguo Chen,
Binguo Chen,
Binguo Chen,
Honghui He,
Honghui He,
Chao He,
Yuanxing Shen,
Yuanxing Shen,
Yuanxing Shen,
Haoyu Zhai,
Haoyu Zhai,
Haoyu Zhai,
Hui Ma,
Hui Ma,
Hui Ma

Affiliations

Conghui Shao: Department of Physics, Tsinghua University, Beijing, China
Conghui Shao: Guangdong Research Center of Polarization Imaging and Measurement Engineering Technology, Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Institute of Optical Imaging and Sensing, Shenzhen International Graduate School, Tsinghua University, Shenzhen, China
Binguo Chen: Guangdong Research Center of Polarization Imaging and Measurement Engineering Technology, Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Institute of Optical Imaging and Sensing, Shenzhen International Graduate School, Tsinghua University, Shenzhen, China
Binguo Chen: Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, China
Binguo Chen: Department of Biomedical Engineering, Tsinghua University, Beijing, China
Honghui He: Guangdong Research Center of Polarization Imaging and Measurement Engineering Technology, Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Institute of Optical Imaging and Sensing, Shenzhen International Graduate School, Tsinghua University, Shenzhen, China
Honghui He: Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, China
Chao He: Department of Engineering Science, University of Oxford, Oxford, United Kingdom
Yuanxing Shen: Guangdong Research Center of Polarization Imaging and Measurement Engineering Technology, Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Institute of Optical Imaging and Sensing, Shenzhen International Graduate School, Tsinghua University, Shenzhen, China
Yuanxing Shen: Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, China
Yuanxing Shen: Department of Biomedical Engineering, Tsinghua University, Beijing, China
Haoyu Zhai: Guangdong Research Center of Polarization Imaging and Measurement Engineering Technology, Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Institute of Optical Imaging and Sensing, Shenzhen International Graduate School, Tsinghua University, Shenzhen, China
Haoyu Zhai: Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, China
Haoyu Zhai: Department of Biomedical Engineering, Tsinghua University, Beijing, China
Hui Ma: Department of Physics, Tsinghua University, Beijing, China
Hui Ma: Guangdong Research Center of Polarization Imaging and Measurement Engineering Technology, Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Institute of Optical Imaging and Sensing, Shenzhen International Graduate School, Tsinghua University, Shenzhen, China
Hui Ma: Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, China

DOI: https://doi.org/10.3389/fchem.2022.936255
Journal volume & issue: Vol. 10

Abstract

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The Mueller matrix contains abundant micro- and even nanostructural information of media. Especially, it can be used as a powerful tool to characterize anisotropic structures quantitatively, such as the particle size, density, and orientation information of fibers in the sample. Compared with unpolarized microscopic imaging techniques, Mueller matrix microscopy can also obtain some essential structural information about the sample from the derived parameters images at low resolution. Here, to analyze the comprehensive effects of imaging resolution on polarization properties obtained from the Mueller matrix, we, first, measure the microscopic Mueller matrices of unstained rat dorsal skin tissue slices rich in collagen fibers using a series of magnifications or numerical aperture (NA) values of objectives. Then, the first-order moments and image texture parameters are quantified and analyzed in conjunction with the polarization parameter images. The results show that the Mueller matrix polar decomposition parameters diattenuation D, linear retardance δ, and depolarization Δ images obtained using low NA objective retain most of the structural information of the sample and can provide fast imaging speed. In addition, the scattering phase function analysis and Monte Carlo simulation based on the cylindrical scatterers reveal that the diattenuation parameter D images with different imaging resolutions are expected to be used to distinguish among the fibrous scatterers in the medium with different particle sizes. This study provides a criterion to decide which structural information can be accurately and rapidly obtained using a transmission Mueller matrix microscope with low NA objectives to assist pathological diagnosis and other applications.

Published in Frontiers in Chemistry

ISSN: 2296-2646 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Chemistry
Website: http://journal.frontiersin.org/journal/chemistry

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