Potential Antioxidant and Wound Healing Effect of Nano-Liposol with High Loading Amount of Astaxanthin

Abstract

Hyeryeon Oh,1,2 Jin Sil Lee,1,2 Daekyung Sung,1 Jong-Min Lim,3,4 Won Il Choi1 1Center for Convergence Bioceramic Materials, Convergence R&D Division, Korea Institute of Ceramic Engineering and Technology, Cheongju, Chungbuk 28160, Republic of Korea; 2School of Materials Science and Engineering and Department of Nanobio Materials and Electronics, Gwangju Institute of Science and Technology, Gwangju 500-712, Republic of Korea; 3Department of Chemical Engineering, Soonchunhyang University, Asan, Chungnam 31538, Republic of Korea; 4Department of Electronic Materials and Devices Engineering, Soonchunhyang University, Asan, Chungnam 31538, Republic of KoreaCorrespondence: Jong-Min Lim Department of Electronic Materials and Devices EngineeringSoonchunhyang University, Asan, Chungnam 31538, Republic of KoreaTel [phone]Fax [phone]Email [email protected] Il ChoiCenter for Convergence Bioceramic Materials, Convergence R&D Division, Korea Institute of Ceramic Engineering and Technology, Cheongju, Chungbuk 28160, Republic of KoreaTel [phone]Fax [phone]Email [email protected]: Astaxanthin (ASTA), a carotenoid, is a strong antioxidant. However, its application in functional foods, pharmaceuticals, and cosmetics remains limited due to its low aqueous solubility and stability. Several different encapsulating materials have been used to improve the stability and bioavailability of ASTA; however, the currently investigated nano-carriers for ASTA require additional improvements with regard to their loading capacity and stability.Methods: In this study, we developed lecithin nano-liposol (Lec NS) as a novel carrier of ASTA using a simple emulsion evaporation method. The physicochemical characteristics including hydrodynamic diameter, polydispersity index, surface charge and morphology were analyzed by DLS and TEM. The antioxidant activity of the ASTA-loaded Lec NS (ASTA@Lec NS) was evaluated using a DPPH radical scavenging assay and in vitro antioxidant assay. The study of in vitro wound healing efficacy was carried out to observe the beneficial effect of antioxidant activity of ASTA@Lec NS on cell migration.Results: ASTA@Lec NS showed improved stability and efficacy owing to improved aqueous solubility of ASTA inside Lec NS. Both in situ and in vitro antioxidant activities of ASTA@Lec NS were higher than that of bare ASTA and Lec NS. It also exhibited strong wound healing efficacy by regulation of ROS level in in vitro cell model.Conclusion: This study revealed that the encapsulation of ASTA into Lec NS using a wet phase transfer enhanced its physiological stability and bioavailability for effective scavenging of reactive oxygen species.Keywords: optimization, lipid micelle, reactive oxygen species, ROS scavenging, cell proliferation

Keywords

• optimization
• lipid micelle
• reactive oxygen species
• ros scavenging
• cell proliferation