Cytotoxic and Antiproliferative Effects of Methanolic Extract of Lavandula Angustifolia on MCF-7 Breast Cancer Cell Line

Abstract

Background and Aim: In recent years, special attention has been paid to the use of complementary drugs in the treatment of breast cancer. Previous studies have shown that lavender (Lavandula angustifolia) has antiproliferative properties. In the present study, the possible antiproliferative effects of methanolic extract of lavender in MCF-7 breast cancer cell line were investigated. Materials and Methods: In this experimental study, MCF-7 breast cancer cell line was treated with different concentrations of methanol extract of lavender. Cell survival was assessed using MTT assay. The cell cycle was analyzed using flow cytometry. The ability of breast cancer cells to form a colony after treatment with plant extract was also investigated. The main components of the methanol extract were identified by GC/MS method. The T-test, ANOVA and Tukey statistical methods were used for data analysis. Results: The results showed that the methanolic extract has significant cytotoxicity on MCF-7 cells, the G0/G1 phase arrest after treatment with methanolic extract in the breast cancer cell line increased significantly. The main contents of the MetOH extract were determined as Coumarin (59.44%), Tricosane (15.22%), 7-methoxy Coumarin (12.69%), and 2-Furancarboxaldehyde (6.7%). Conclusion: In conclusion, according to the results of this study, the lavender methanolic extract has a cytotoxic effect on MCF-7 cells; therefore, lavender can be considered as a potential therapeutic supplement in future studies of breast cancer.

Keywords

• cytotoxicity
• breast cancer
• lavandula angustifolia
• methanolic extract
• mcf-7