Quantification of purified endogenous miRNAs with high sensitivity and specificity

Soochul Shin; Yoonseok Jung; Heesoo Uhm; Minseok Song; Soomin Son; Jiyoung Goo; Cherlhyun Jeong; Ji-Joon Song; V. Narry Kim; Sungchul Hohng

doi:10.1038/s41467-020-19865-9

Nature Communications (Nov 2020)

Quantification of purified endogenous miRNAs with high sensitivity and specificity

Soochul Shin,
Yoonseok Jung,
Heesoo Uhm,
Minseok Song,
Soomin Son,
Jiyoung Goo,
Cherlhyun Jeong,
Ji-Joon Song,
V. Narry Kim,
Sungchul Hohng

Affiliations

Soochul Shin: Department of Physics and Astronomy, and Institute of Applied Physics, Seoul National University
Yoonseok Jung: Center for RNA Research, Institute for Basic Science (IBS)
Heesoo Uhm: Department of Physics and Astronomy, and Institute of Applied Physics, Seoul National University
Minseok Song: Department of Physics and Astronomy, and Institute of Applied Physics, Seoul National University
Soomin Son: Center for RNA Research, Institute for Basic Science (IBS)
Jiyoung Goo: Center for Theragnosis, Korea Institute of Science and Technology
Cherlhyun Jeong: Center for Theragnosis, Korea Institute of Science and Technology
Ji-Joon Song: Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST)
V. Narry Kim: Center for RNA Research, Institute for Basic Science (IBS)
Sungchul Hohng: Department of Physics and Astronomy, and Institute of Applied Physics, Seoul National University

DOI: https://doi.org/10.1038/s41467-020-19865-9
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 8

Abstract

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MicroRNAs are potentially powerful biomarkers, though clinical use requires rapid and reliable profiling. Here the authors report amplification-free multicolour single-molecule imaging with single base mismatch sensitivity.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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