LncRNA-ENST00000421645 Upregulates Kank1 to Inhibit IFN-γ Expression and Promote T Cell Apoptosis in Neurosyphilis

Kai-Xuan Wu; Xiao-Tong Wang; Xin-Lin Hu; Xiao-Yong Jiang; Jing-Cong Zhuang; Yan-Zhu Xu; Li-Rong Lin; Man-Li Tong; Tian-Ci Yang; Li-Li Liu

doi:10.3389/fmicb.2021.749171

Frontiers in Microbiology (Nov 2021)

LncRNA-ENST00000421645 Upregulates Kank1 to Inhibit IFN-γ Expression and Promote T Cell Apoptosis in Neurosyphilis

Kai-Xuan Wu,
Xiao-Tong Wang,
Xin-Lin Hu,
Xiao-Yong Jiang,
Jing-Cong Zhuang,
Yan-Zhu Xu,
Li-Rong Lin,
Man-Li Tong,
Tian-Ci Yang,
Li-Li Liu

Affiliations

Kai-Xuan Wu: Center of Clinical Laboratory, School of Medical, Zhongshan Hospital, Xiamen University, Xiamen, China
Xiao-Tong Wang: Center of Clinical Laboratory, School of Medical, Zhongshan Hospital, Xiamen University, Xiamen, China
Xin-Lin Hu: Department of Dermatology, School of Medical, Zhongshan Hospital, Xiamen University, Xiamen, China
Xiao-Yong Jiang: Department of Dermatology, School of Medical, Zhongshan Hospital, Xiamen University, Xiamen, China
Jing-Cong Zhuang: Department of Neurology, School of Medical, Zhongshan Hospital, Xiamen University, Xiamen, China
Yan-Zhu Xu: Department of Dermatology, School of Medical, Zhongshan Hospital, Xiamen University, Xiamen, China
Li-Rong Lin: Center of Clinical Laboratory, School of Medical, Zhongshan Hospital, Xiamen University, Xiamen, China
Man-Li Tong: Center of Clinical Laboratory, School of Medical, Zhongshan Hospital, Xiamen University, Xiamen, China
Tian-Ci Yang: Center of Clinical Laboratory, School of Medical, Zhongshan Hospital, Xiamen University, Xiamen, China
Li-Li Liu: Center of Clinical Laboratory, School of Medical, Zhongshan Hospital, Xiamen University, Xiamen, China

DOI: https://doi.org/10.3389/fmicb.2021.749171
Journal volume & issue: Vol. 12

Abstract

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Long non-coding RNAs are involved in many infectious diseases. Our previous studies showed that lncRNA-ENST00000421645 expression is increased in T lymphocytes of neurosyphilis patients compared to healthy controls. However, whether lncRNA-ENST00000421645 has biological functions remains unclear. The current study was undertaken to understand the mechanism of lncRNA-ENST00000421645 in T lymphocyte function in neurosyphilis patients. The lncRNA-ENST00000421645 pull-down assay showed that lncRNA-ENST00000421645 acted on the acetylase NAT10. The chromatin immunoprecipitation (ChIP)-PCR results showed that lncRNA-ENST00000421645 promoted the acetylation of histone H3K27 adjacent to the Kank1 promoter, thereby promoting Kank1 protein expression. Kank1 promotes 14-3-3 protein expression, inhibits NF-kB activation, inhibits IFN-γ secretion by T lymphocytes, and promotes T lymphocyte apoptosis. Taken together, our findings suggest a novel mechanism that LncRNA-ENST00000421645 upregulates Kank1 to inhibit IFN-γ expression and promote T cell apoptosis in neurosyphilis.

Published in Frontiers in Microbiology

ISSN: 1664-302X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.frontiersin.org/journals/microbiology

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