Prospects for Using Atomic Power Microscopic Methods in Reanimatology

Abstract

Objective: to obtain an image of pores in the membrane of an erythrocyte after its electroporation, by using an atomic power microscope (APM), to evaluate the parameters of the obtained pores, and to discuss the prospects for using an APM in reanimatology. Human whole venous blood was exposed to a pulsed electric field, by inducing erythrocytic membrane elec-troporation. Blood smears were obtained by the standard method. Images of the form and surface of individual erythrocytes and their enlarged fragments were obtained by means of a Femto Scan APM. The diameters, depths, and configuration of some pores were analyzed. This example and others were used to show a wide field and prospects for using an APM in reanimatology.

Keywords

• атомный силовой микроскоп
• электропорация мембран
• анализ пор.