Screening of Medium Compositions for Recombinant Lipase Production via Two-level Fractional Factorial Design

Abstract

The increasing demand for natural producing protein such as lipase in the food and detergent industries had been in great concern for the past decades. Therefore, the production of recombinant lipase may serve as an alternative. A two-level fractional factorial design approach was carried out to measure and screen the effect of five medium components as the factors, namely ammonium sulphate ((NH4)2SO4), glycerol, yeast extract, monopotassium dihydrogen phosphate (KH2PO4) and dipotassium phosphate (K2HPO4). The factors were exploited in low and high-level parameter ranges, reflected by 16 experiments conducted in shake flasks of recombinant E. coli (rE. coli). Factors and interactions between the two factors found below than p<0.05 significantly contributed to the lipase production and total protein. For the lipase, (NH4)2SO4 and K2HPO4 were the significant factors while for the total protein, only K2HPO4 was found significant. The interactions between the factors, however, varied considerably. This experimental design was also providing Pareto analysis to understand the positive and negative contributions of the respective factor and the interactions of the factors.

Keywords

• : fractional factorial design
• recombinant e. coli
• recombinant lipase
• two-level fractional factorial design