Genome-wide comparison and identification of myosin gene family in Arabidopsis thaliana and Helianthus annuus
Hafiz Muhammad Ahmad,
Hayat Ali Alafari,
Sajid Fiaz,
Dalal S. Alshaya,
Sidra Toor,
Munazza Ijaz,
Nouman Rasool,
Kotb A. Attia,
Madiha Zaynab,
Saira Azmat,
Asmaa M. Abushady,
Yinglong Chen
Affiliations
Hafiz Muhammad Ahmad
Department of Bioinformatics and Biotechnology, Government College University Faisalabad, Pakistan; Corresponding author.
Hayat Ali Alafari
Deparment of Biology, College of Science, Princess Nourah Bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia
Sajid Fiaz
Department of Plant Breeding and Genetics, University of Haripur, Haripur 22620, Pakistan; Corresponding author.
Dalal S. Alshaya
Deparment of Biology, College of Science, Princess Nourah Bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia
Sidra Toor
Department of Life Sciences, University of Management and Technology, Lahore, Pakistan
Munazza Ijaz
State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou, 310058, China
Nouman Rasool
Department of Plant Breeding and Genetics, University of Haripur, Haripur 22620, Pakistan
Kotb A. Attia
Center of Excellence in Biotechnology Research, King Saud University, P.O. Box 2455-11451, Riyadh 11451, Saudi Arabia; Department of Rice Biotechnology, RRTC, Institute of Field Crops, ARC, Sakha, 33177, Kafrelsheikh, Egypt
Madiha Zaynab
College of Life Science & Oceanography, Shenzhen University, China
Saira Azmat
Agriculture Extension and Adaptive Research, Agriculture Department, Government of Punjab, Pakistan
Asmaa M. Abushady
Biotechnology School, Nile University, 26th of July Corridor, Sheikh Zayed City, Giza, 12588, Egypt; Department of Genetics, Agriculture College, Ain Shams University, Cairo, Egypt
Yinglong Chen
School of Earth and Environment and UWA Institute of Agriculture, University of Western Australia, Australia
Myosins are essential components of organelle trafficking in all the eukaryotic cells. Myosin driven movement plays a vital role in the development of pollen tubes, root hairs and root tips of flowering plants. The present research characterized the myosin genes in Arabidopsis thaliana and Helianthus annuus by using different computational tools. We discovered a total of 50 myosin genes and their splice variants in both pant species. Phylogenetic analysis indicated that myosin genes were divided into four subclasses. Chromosomal location revealed that myosin genes were located on all five chromosomes in A. thaliana, whereas they were present on nine chromosomes in H. annuus. Conserved motifs showed that conserved regions were closely similar within subgroups. Gene structure analysis showed that Atmyosin2.2 and Atmyosin2.3 had the highest number of introns/exons. Gene ontology analysis indicated that myosin genes were involved in vesicle transport along actin filament and cytoskeleton trafficking. Expression analysis showed that expression of myosin genes was higher during the flowering stage as compared to the seedling and budding stages. Tissue specific expression indicated that HanMYOSIN11.2, HanMYOSIN16.2 were highly expressed in stamen, whereas HanMYOSIN 2.2, HanMYOSIN 12.1 and HanMYOSIN 17.1 showed higher expression in nectary. This study enhance our understanding the function of myosins in plant development, and forms the basis for future research about the comparative genomics of plant myosin in other crop plants.
