Molecular Characterisation of Uterine Endometrial Proteins during Early Stages of Pregnancy in Pigs by MALDI TOF/TOF

Dorota Pierzchała; Kamila Liput; Agnieszka Korwin-Kossakowska; Magdalena Ogłuszka; Ewa Poławska; Agata Nawrocka; Paweł Urbański; Aleksandra Ciepłoch; Edyta Juszczuk-Kubiak; Adam Lepczyński; Brygida Ślaska; Krzysztof Kowal; Marinus F. W. te Pas; Magdalena Śmiech; Paweł Leszczyński; Hiroaki Taniguchi; Leyland Fraser; Przemysław Sobiech; Mateusz Sachajko; Magdalena Herudzinska; Chandra S. Pareek; Mariusz Pierzchała

doi:10.3390/ijms22136720

International Journal of Molecular Sciences (Jun 2021)

Molecular Characterisation of Uterine Endometrial Proteins during Early Stages of Pregnancy in Pigs by MALDI TOF/TOF

Dorota Pierzchała,
Kamila Liput,
Agnieszka Korwin-Kossakowska,
Magdalena Ogłuszka,
Ewa Poławska,
Agata Nawrocka,
Paweł Urbański,
Aleksandra Ciepłoch,
Edyta Juszczuk-Kubiak,
Adam Lepczyński,
Brygida Ślaska,
Krzysztof Kowal,
Marinus F. W. te Pas,
Magdalena Śmiech,
Paweł Leszczyński,
Hiroaki Taniguchi,
Leyland Fraser,
Przemysław Sobiech,
Mateusz Sachajko,
Magdalena Herudzinska,
Chandra S. Pareek,
Mariusz Pierzchała

Affiliations

Dorota Pierzchała: Department of Animal Genomics and Biodiversity, Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Jastrzebiec, ul. Postepu 36A, 05-552 Magdalenka, Poland
Kamila Liput: Department of Animal Genomics and Biodiversity, Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Jastrzebiec, ul. Postepu 36A, 05-552 Magdalenka, Poland
Agnieszka Korwin-Kossakowska: Department of Animal Genomics and Biodiversity, Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Jastrzebiec, ul. Postepu 36A, 05-552 Magdalenka, Poland
Magdalena Ogłuszka: Department of Animal Genomics and Biodiversity, Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Jastrzebiec, ul. Postepu 36A, 05-552 Magdalenka, Poland
Ewa Poławska: Department of Animal Genomics and Biodiversity, Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Jastrzebiec, ul. Postepu 36A, 05-552 Magdalenka, Poland
Agata Nawrocka: Department of Animal Genomics and Biodiversity, Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Jastrzebiec, ul. Postepu 36A, 05-552 Magdalenka, Poland
Paweł Urbański: Department of Animal Genomics and Biodiversity, Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Jastrzebiec, ul. Postepu 36A, 05-552 Magdalenka, Poland
Aleksandra Ciepłoch: Department of Animal Genomics and Biodiversity, Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Jastrzebiec, ul. Postepu 36A, 05-552 Magdalenka, Poland
Edyta Juszczuk-Kubiak: Laboratory of Biotechnology and Molecular Engineering, Department of Microbiology, Prof. Wacław Dąbrowski Institute of Agricultural and Food Biotechnology, State Research Institute, Rakowiecka 36 Street, 02-532 Warsaw, Poland
Adam Lepczyński: Department of Physiology, Cytobiology and Proteomics, West Pomeranian University of Technology, ul. K. Janickiego 32, 71-270 Szczecin, Poland
Brygida Ślaska: Faculty of Animal Sciences and Bioeconomy, Institute of Biological Bases of Animal Production, University of Life Sciences in Lublin, ul. Akademicka 13, 20-950 Lublin, Poland
Krzysztof Kowal: Faculty of Animal Sciences and Bioeconomy, Institute of Biological Bases of Animal Production, University of Life Sciences in Lublin, ul. Akademicka 13, 20-950 Lublin, Poland
Marinus F. W. te Pas: The Netherlands Wageningen UR Livestock Research, Animal Breeding and Genomics, P.O. Box 9101, 6700 HB Wageningen, The Netherlands
Magdalena Śmiech: Department of Experimental Embryology, Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzebiec, ul. Postepu 36A, 05-552 Magdalenka, Poland
Paweł Leszczyński: Department of Experimental Embryology, Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzebiec, ul. Postepu 36A, 05-552 Magdalenka, Poland
Hiroaki Taniguchi: Department of Experimental Embryology, Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzebiec, ul. Postepu 36A, 05-552 Magdalenka, Poland
Leyland Fraser: Department of Animal Biochemistry and Biotechnology, Faculty of Animal Bio-Engineering, University of Warmia and Mazury in Olsztyn, ul. Oczapowskiego 5, 10-719 Olsztyn, Poland
Przemysław Sobiech: Internal Disease Unit, Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland
Mateusz Sachajko: Faculty of Biological and Veterinary Sciences, Institute of Veterinary Medicine, Nicolaus Copernicus University, ul. Gagarina 7, 87-100 Toruń, Poland
Magdalena Herudzinska: Faculty of Biological and Veterinary Sciences, Institute of Veterinary Medicine, Nicolaus Copernicus University, ul. Gagarina 7, 87-100 Toruń, Poland
Chandra S. Pareek: Faculty of Biological and Veterinary Sciences, Institute of Veterinary Medicine, Nicolaus Copernicus University, ul. Gagarina 7, 87-100 Toruń, Poland
Mariusz Pierzchała: Department of Animal Genomics and Biodiversity, Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Jastrzebiec, ul. Postepu 36A, 05-552 Magdalenka, Poland

DOI: https://doi.org/10.3390/ijms22136720
Journal volume & issue: Vol. 22, no. 13
p. 6720

Abstract

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The molecular mechanism underlying embryonic implantation is vital to understand the correct communications between endometrium and developing conceptus during early stages of pregnancy. This study’s objective was to determine molecular changes in the uterine endometrial proteome during the preimplantation and peri-implantation between 9 days (9D), 12 days (12D), and 16 days (16D) of pregnant Polish Large White (PLW) gilts. 2DE-MALDI-TOF/TOF and ClueGOTM approaches were employed to analyse the biological networks and molecular changes in porcine endometrial proteome during maternal recognition of pregnancy. A total of sixteen differentially expressed proteins (DEPs) were identified using 2-DE gels and MALDI-TOF/TOF mass spectrometry. Comparison between 9D and 12D of pregnancy identified APOA1, CAPZB, LDHB, CCT5, ANXA4, CFB, TTR upregulated DEPs, and ANXA5, SMS downregulated DEPs. Comparison between 9D and 16D of pregnancy identified HP, APOA1, ACTB, CCT5, ANXA4, CFB upregulated DEPs and ANXA5, SMS, LDHB, ACTR3, HP, ENO3, OAT downregulated DEPs. However, a comparison between 12D and 16D of pregnancy identified HP, ACTB upregulated DEPs, and CRYM, ANXA4, ANXA5, CAPZB, LDHB, ACTR3, CCT5, ENO3, OAT, TTR down-regulated DEPs. Outcomes of this study revealed key proteins and their interactions with metabolic pathways involved in the recognition and establishment of early pregnancy in PLW gilts.

Published in International Journal of Molecular Sciences

ISSN: 1661-6596 (Print); 1422-0067 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Biology (General); Science: Chemistry
Website: http://www.mdpi.com/journal/ijms

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