The influence of Schiff base inclusion complexes with β-cyclodextrine on antibiotic production by Streptomyces hygroscopicus CH-7

Abstract

A media consisting of isatin-Schiff bases and its inclusion complexes with β-cyclodextrine was developed to maximize the production of antibiotics Hexaene H-85 and Azalomycine β by Streptomyces hygroscopicus CH-7. The media with β-cyclodextrine inclusion complex of isatin-3-thiosemicarbazone resulted in the maximum antibiotics concentration of 493 µg/cm3 for Hexaene H-85 and 191 µg/cm3 for Azalomycine B. The production of Hexaene H-85 and Azalomycine is higher when β-cyclodextrine complex is added as a nitrogen source, comparing to pure isatin-Schiff base. The maximum concentration of hexaene H-85 in medium with inclusion complex of isatin-Schiff base is 1.4-2.3 times higher than the basal medium. The maximum production of Azalomycine is 2.1-3.4 times higher in media with inclusion complex of Schiff base. During the fermentation process, the nutrient media with β-cyclodextrine inclusion complexes with isatin-Schiff bases affect the strain morphology, since it is in the form of compact pellets, which are formed from short and long, branched filaments [Projekat Ministarstva nauke Republike Srbije, br. III 45001]

Keywords

• Streptomyces hygroscopicus
• Schiff base
• β-cyclodextrine
• inclusion complex
• antibiotic production