Antiproliferative Activity and Induction of Apoptosis in PC-3 Cells by the Chalcone Cardamonin from Campomanesia adamantium (Myrtaceae) in a Bioactivity-Guided Study
Aislan Cristina Rheder Fagundes Pascoal,
Carlos Augusto Ehrenfried,
Begoña Gimenez-Cassina Lopez,
Thiago Matos de Araujo,
Vinicius D’ávila Bitencourt Pascoal,
Rovilson Gilioli,
Gabriel Forato Anhê,
Ana Lúcia Tasca Goes Ruiz,
João Ernesto de Carvalho,
Maria Élida Alves Stefanello,
Marcos José Salvador
Affiliations
Aislan Cristina Rheder Fagundes Pascoal
Graduate Program in Biosciences and Technology of Bioactive Products, Pharmacy course, State University of Campinas, Campinas, 6109, São Paulo 13083-970, Brazil
Carlos Augusto Ehrenfried
Department of Chemistry, Federal University of Paraná, 19081, Curitiba 81531-990, Paraná, Brazil
Begoña Gimenez-Cassina Lopez
Graduate Program in Biosciences and Technology of Bioactive Products, Pharmacy course, State University of Campinas, Campinas, 6109, São Paulo 13083-970, Brazil
Thiago Matos de Araujo
Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas, Campinas 13083-887, São Paulo, Brazil
Vinicius D’ávila Bitencourt Pascoal
Faculty of Basic Sciences, Fluminense Federal University, Nova Friburgo 24020-420, Rio de Janeiro, Brazil
Rovilson Gilioli
Multidisciplinary Center for Biological Research, Laboratory Animal Quality Control, State University of Campinas, l6095, Campinas 13083-877, São Paulo, Brazil
Gabriel Forato Anhê
Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas, Campinas 13083-887, São Paulo, Brazil
Ana Lúcia Tasca Goes Ruiz
Division of Pharmacology and Toxicology, Multidisciplinary Center for Chemical, Biological and Agricultural, State University of Campinas, 6171, Campinas 13081-970, São Paulo, Brazil
João Ernesto de Carvalho
Division of Pharmacology and Toxicology, Multidisciplinary Center for Chemical, Biological and Agricultural, State University of Campinas, 6171, Campinas 13081-970, São Paulo, Brazil
Maria Élida Alves Stefanello
Department of Chemistry, Federal University of Paraná, 19081, Curitiba 81531-990, Paraná, Brazil
Marcos José Salvador
Graduate Program in Biosciences and Technology of Bioactive Products, Pharmacy course, State University of Campinas, Campinas, 6109, São Paulo 13083-970, Brazil
The Myrtaceae family is a common source of medicines used in the treatment of numerous diseases in South America. In Brazil, fruits of the Campomanesia species are widely used to make liqueurs, juices and sweets, whereas leaves are traditionally employed as a medicine for dysentery, stomach problems, diarrhea, cystitis and urethritis. Ethanol extracts of Campomanesia adamantium (Myrtaceae) leaves and fruits were evaluated against prostate cancer cells (PC-3). The compound (2E)-1-(2,4-dihydroxy-6-methoxyphenyl)-3-phenylprop-2-en-1-one, cardamonin) was isolated from ethanol extracts of C. adamantium leaves in a bioactivity-guided study and quantified by UPLC-MS/MS. In vitro studies showed that the isolated chalcone cardamonin inhibited prostate cancer cell proliferation and decreased the expression of NFkB1. Moreover, analysis by flow cytometry showed that this compound induced DNA fragmentation, suggesting an effect on apoptosis induction in the PC-3 cell line.
