Utilisation of SSRs for Characterisation of the Soybean (Glycine max (L.) Merr.) Genetic Resources

Abstract

The SSR profiles of 67 soybean genotypes of various origins have been detected by 188 alleles at the 18 SSR loci. From 4 to 21 alleles were found at each of these loci (average 10.4 per locus) and the gene diversity averaged 0.71. Differentiation of all 67 genotypes each from others has been successful by using of even only 6 of SSR markers (Sat001, Satt005, Satt038, Satt173, Satt177, Satt534) with gene diversity from 0.66 to 0.89. The cumulated probability of obtaining identical soybean SSR profile was 1.11 × 10-9 , which confirms a high potential of SSRs for differentiation of soybean accessions in collections. Clustering of genotypes partially reflects origin and pedigree of analysed soybean accessions.

Keywords

• soybean
• glycine max (l.) merr.
• microsatellite
• genetic diversity