Patologìâ (Dec 2018)
Immunogenetic factors for the development of premature rupture of membranes in preterm labour in Zaporizhzhia region
Abstract
Aim. To study single nucleotide polymorphism of proinflmmatory cytokine genes IL1β (rs1143627) and TNFα (rs1800629) and determine mRNA level of IL1β IL17A genes at the local level in PROM during 26–34 weeks of pregnancy in the population of Zaporizhzhia region. Materials and methods. Cytokine gene markers were studied in 50 women with PROM in gestational age of 26–34 weeks and 50 pregnant women with physiological pregnancy and term labor without complications. Genotyping with TaqMan samples was performed on CFX96 ™ Real-Time PCR Detection Systems amplifir (Bio-RadLaboratories, Inc, USA). For determination of mRNA level of proinflmmatory cytokine genes IL1β (NM_000576.2) and IL17A (NM_002190.2) PCR in real time and the Luminaris HiGreen Fluorescein qPCR MasterMix reagent kit (ThermoScientifi, USA) were used. Results. We were unable to identify statistically signifiant diffrences for each of the rs1143627 polymorphism genotypes (GG, GA and AA) of the IL1β gene between the main study group and the control group (χ2 = 0.18, OR (GG) = 1.13; 95 % CI: 0.65–1.98; OR (GA) = 1.54; 95 % CI: 0.68–3.49; and OR (AA) = 0.89; 95 % CI: 0.50–1.55, respectively, P > 0.05). Between the main and control groups, including all alleles of the rs1800629 polymorphism (AA, GG and AG) of the TNFα gene there was a tendency that didn’t become of statistical signifiance – χ2 = 0.44, OR (AA) = 0.8; 95 % CI: 0.42–1.54; OR (GG) = 1.25; 95 % CI: 0.65–2.39; and OR (AG) = 0.67; 95 % CI: 0.28–1.62, respectively, P < 0.05. The range of all obtained values of the relative normalized expression of IL1β gene mRNA in placenta was 1.43–227.93 (mean – 25.08), in fetal membranes – 1.23–139.24 (mean – 23.83). The range of relative normalized expression of mRNA of the IL-17A gene in placenta was 1.15–62.76 (mean – 5.69), in fetal membranes – 1.63–130.67 (mean – 19.31). Conclusions. In the population of Zaporizhzhia region, there is no reliable clinical association between the IL1β (rs1143627) and TNFα (rs1800629) genes and a high risk of developing premature rupture of the membranes in preterm pregnancy. PCR in real time allowed to identify the immune-mediated level of the development of PROM at the local level in the gestation period of 26-34 weeks as great increased genes transcriptional activity of pro-inflmmatory cytokines IL1β and IL17A.
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