Frontiers in Microbiology (Dec 2020)
Probiotic Lactobacillus rhamnosus GG Promotes Mouse Gut Microbiota Diversity and T Cell Differentiation
- Chun-wei Shi,
- Chun-wei Shi,
- Chun-wei Shi,
- Ming-yang Cheng,
- Ming-yang Cheng,
- Ming-yang Cheng,
- Xin Yang,
- Xin Yang,
- Xin Yang,
- Yi-yuan Lu,
- Yi-yuan Lu,
- Yi-yuan Lu,
- Hong-duo Yin,
- Hong-duo Yin,
- Hong-duo Yin,
- Yan Zeng,
- Yan Zeng,
- Yan Zeng,
- Ru-yu Wang,
- Ru-yu Wang,
- Ru-yu Wang,
- Yan-long Jiang,
- Yan-long Jiang,
- Yan-long Jiang,
- Wen-tao Yang,
- Wen-tao Yang,
- Wen-tao Yang,
- Jian-zhong Wang,
- Jian-zhong Wang,
- Jian-zhong Wang,
- Dan-dan Zhao,
- Dan-dan Zhao,
- Dan-dan Zhao,
- Hai-bin Huang,
- Hai-bin Huang,
- Hai-bin Huang,
- Li-ping Ye,
- Li-ping Ye,
- Li-ping Ye,
- Xin Cao,
- Xin Cao,
- Xin Cao,
- Gui-lian Yang,
- Gui-lian Yang,
- Gui-lian Yang,
- Chun-feng Wang,
- Chun-feng Wang,
- Chun-feng Wang
Affiliations
- Chun-wei Shi
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Chun-wei Shi
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Chun-wei Shi
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Ming-yang Cheng
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Ming-yang Cheng
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Ming-yang Cheng
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Xin Yang
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Xin Yang
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Xin Yang
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Yi-yuan Lu
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Yi-yuan Lu
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Yi-yuan Lu
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Hong-duo Yin
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Hong-duo Yin
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Hong-duo Yin
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Yan Zeng
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Yan Zeng
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Yan Zeng
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Ru-yu Wang
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Ru-yu Wang
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Ru-yu Wang
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Yan-long Jiang
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Yan-long Jiang
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Yan-long Jiang
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Wen-tao Yang
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Wen-tao Yang
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Wen-tao Yang
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Jian-zhong Wang
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Jian-zhong Wang
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Jian-zhong Wang
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Dan-dan Zhao
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Dan-dan Zhao
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Dan-dan Zhao
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Hai-bin Huang
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Hai-bin Huang
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Hai-bin Huang
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Li-ping Ye
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Li-ping Ye
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Li-ping Ye
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Xin Cao
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Xin Cao
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Xin Cao
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Gui-lian Yang
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Gui-lian Yang
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Gui-lian Yang
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Chun-feng Wang
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Chun-feng Wang
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Chun-feng Wang
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- DOI
- https://doi.org/10.3389/fmicb.2020.607735
- Journal volume & issue
-
Vol. 11
Abstract
Lactic acid bacteria (LAB) are the primary genera of the intestinal flora and have many probiotic functions. In the present study, Lactobacillus rhamnosus GG (LGG) ATCC 53103 was used to treat BALB/c mice. After LGG intervention, both low and high LGG doses were shown to improve the observed OTU, Chao1, ACE, and Shannon indices, while the Simpson index decreased, demonstrating that LGG can promote intestinal microbiota abundance and diversity. Furthermore, LGG treatment increased the abundances of intestinal Firmicutes, Bacteroides and Actinomycetes while reducing that of Proteobacteria. In addition to its effect on gut the microbiota, LGG could also regulate the host immune system. In the present study, we showed that LGG could affect the percentage of CD3+ T lymphocytes in the spleens (SPLs), mesenteric lymph nodes (MLNs), Peyer’s patches (PPs) and lamina propria lymphocytes (LPLs) of mice, including total CD3+ T, CD3+CD4+ T, and CD3+CD8+ T lymphocytes. Furthermore, LGG could effectively increase the expression of Th1-type cytokines (IFN-γ) and Th2 cytokines (IL-4) in CD4+ T cells, indicating that the proportion of Th1 and Th2 cells in mice with LGG treatment was in a high equilibrium state compared to the control group. In addition, the IFN-γ/IL-4 ratio was greater than 1 in mice with LGG intervention, suggesting that LGG tends to mediate the Th1 immune response. The results of the present study also showed that LGG upregulated the expression of IL-17 in CD4+ T cells and regulated the percentage of CD4+CD25+Foxp3+ Treg cells in various secondary immunological organs, indicating that LGG may promote the balance of Th-17 and Treg cells.
Keywords
