Endocrine Connections (May 2020)

Genetic background influences tumour development in heterozygous Men1 knockout mice

  • Kate E Lines,
  • Mahsa Javid,
  • Anita A C Reed,
  • Gerard V Walls,
  • Mark Stevenson,
  • Michelle Simon,
  • Kreepa G Kooblall,
  • Sian E Piret,
  • Paul T Christie,
  • Paul J Newey,
  • Ann-Marie Mallon,
  • Rajesh V Thakker

DOI
https://doi.org/10.1530/EC-20-0103
Journal volume & issue
Vol. 9, no. 5
pp. 426 – 437

Abstract

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Multiple endocrine neoplasia type 1 (MEN1), an autosomal domina nt disorder caused by MEN1 germline mutations, is characterised by parathyroid, pancreati c and pituitary tumours. MEN1 mutations also cause familial isolated primary hyperparathyroi dism (FIHP), a milder condition causing hyperparathyroidism only. Identical mutations can cause either MEN1 or FIHP in different families, thereby implicating a role for genetic modifiers in altering phenotypic expression of tumours. We therefore inve stigated the effects of genetic background and potential for genetic modifiers on tumour development in adult Men1+/- mice, which develop tumours of the parathyroids, pancreatic is lets, anterior pituitary, adrenal cortex and gonads, that had been backcrossed to generate C57BL/6 and 129S6/SvEv congenic strains. A total of 275 Men1+/- mice, aged 5–26 months were macroscopically studied, and this revealed that genetic backgro und significantly influenced the development of pituitary, adrenal and ovarian tumours, whic h occurred in mice over 12 months of age and more frequently in C57BL/6 females, 129S6/ SvEv males and 129S6/SvEv females, respectively. Moreover, pituitary and adrenal tumours developed earlier, in C57BL/6 males and 129S6/SvEv females, respectively, and pancreatic and testicular tumours developed earlier in 129S6/SvEv males. Furth ermore, glucagon-positive staining pancreatic tumours occurred more frequently in 129S6/S vEv Men1+/- mice. Whole genome sequence analysis of 129S6/SvEv and C57BL/6 Men1+/- mice revealed >54,000 different variants in >300 genes. These included, Coq7, Dmpk, Ccne2, Kras, Wnt2b, Il3ra and Tnfrsf10a, and qRT-PCR analysis revealed that Kras was significantly higher in pituitaries of male 129S6/SvEv mice. Thus, our results demonstrate that Kras and other genes could represent possible genetic modifiers of Men1.

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