Modulation of rice grain shape and appearance by the GS10-encoded long coiled-coil protein
Yanmin Luo,
Yuyu Chen,
Pao Xue,
Beifang Wang,
Yiwei Kang,
Yue Zhang,
Daibo Chen,
Yongbo Hong,
Weixun Wu,
Qunen Liu,
Xiaodeng Zhan,
Yongjun Lin,
Shihua Cheng,
Yingxin Zhang,
Liyong Cao
Affiliations
Yanmin Luo
State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, Zhejiang, China; Northern Rice Research Center of Bao Qing, Shuangyashan 155600, Heilongjiang, China; Zhejiang Key Laboratory of Super Rice Research, China National Rice Research Institute, Hangzhou 311400, Zhejiang, China
Yuyu Chen
State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, Zhejiang, China; National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, Hubei, China; Northern Rice Research Center of Bao Qing, Shuangyashan 155600, Heilongjiang, China
Pao Xue
State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, Zhejiang, China
Beifang Wang
State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, Zhejiang, China
Yiwei Kang
State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, Zhejiang, China; National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, Hubei, China
Yue Zhang
State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, Zhejiang, China
Daibo Chen
State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, Zhejiang, China; Zhejiang Key Laboratory of Super Rice Research, China National Rice Research Institute, Hangzhou 311400, Zhejiang, China
Yongbo Hong
State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, Zhejiang, China; National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya 572025, Hainan, China
Weixun Wu
State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, Zhejiang, China
Qunen Liu
State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, Zhejiang, China
Xiaodeng Zhan
State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, Zhejiang, China; Zhejiang Key Laboratory of Super Rice Research, China National Rice Research Institute, Hangzhou 311400, Zhejiang, China
Yongjun Lin
National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, Hubei, China
Shihua Cheng
State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, Zhejiang, China
Yingxin Zhang
State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, Zhejiang, China; Corresponding author.
Liyong Cao
State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, Zhejiang, China; Northern Rice Research Center of Bao Qing, Shuangyashan 155600, Heilongjiang, China; National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya 572025, Hainan, China; Zhejiang Key Laboratory of Super Rice Research, China National Rice Research Institute, Hangzhou 311400, Zhejiang, China; Corresponding author.
Rice grain size is a primary characteristic essential for artificial domestication and breeding, governed by grain length, width, and thickness. In this study, we cloned Grain Size 10 (GS10), a novel gene via map-based cloning. Biochemical, molecular, and genetic studies were performed to elucidate the GS10 involved grain size mechanism in rice. Mutant of GS10 lead to reduced grain size due to alterations in cell expansion. Additionally, GS10 is responsible for the formation of notched-belly grains, especially in smaller grain varieties possessing loss-function mutations. Overexpression of GS10 in Nipponbare results in increasing grain length, grain weight and improve the appearance quality of rice. GS10 encodes conserved protein with uncharacterized function. Furthermore, GS10 regulates the grain size by interacting OsBRICK1, a subunit of the WAVE complex that governs actin nucleation and affects the assembly of microfilaments in rice. Together, our study demonstrates that, GS10 positively regulates the grain length and grain weight, which is beneficial for further improvements in yield characteristics.
