Development and Characterization of PEGDA Microneedles for Localized Drug Delivery of Gemcitabine to Treat Inflammatory Breast Cancer
Ahmed Alafnan,
Aravindram Attiguppe Seetharam,
Talib Hussain,
Maram Suresh Gupta,
Syed Mohd Danish Rizvi,
Afrasim Moin,
Abdulwahab Alamri,
Aziz Unnisa,
Amir Mahgoub Awadelkareem,
AbdElmoneim O. Elkhalifa,
Pradyumna Jayahanumaiah,
Mohammad Khalid,
Natchimuthu Balashanmugam
Affiliations
Ahmed Alafnan
Department of Pharmacology and Toxicology, College of Pharmacy, University of Ha’il, Ha’il 81442, Saudi Arabia
Aravindram Attiguppe Seetharam
Department of Pharmaceutics, JSS College of Pharmacy, JSS Academy of Higher Education and Research (JSSAHER), Sri Shivarathreeshwara Nagar, Mysore 570015, India
Talib Hussain
Department of Pharmacology and Toxicology, College of Pharmacy, University of Ha’il, Ha’il 81442, Saudi Arabia
Maram Suresh Gupta
Department of Pharmaceutics, JSS College of Pharmacy, JSS Academy of Higher Education and Research (JSSAHER), Sri Shivarathreeshwara Nagar, Mysore 570015, India
Syed Mohd Danish Rizvi
Department of Pharmaceutics, College of Pharmacy, University of Ha’il, Ha’il 81442, Saudi Arabia
Afrasim Moin
Department of Pharmaceutics, College of Pharmacy, University of Ha’il, Ha’il 81442, Saudi Arabia
Abdulwahab Alamri
Department of Pharmacology and Toxicology, College of Pharmacy, University of Ha’il, Ha’il 81442, Saudi Arabia
Aziz Unnisa
Department of Pharmaceutical Chemistry, College of Pharmacy, University of Ha’il, Ha’il 81442, Saudi Arabia
Amir Mahgoub Awadelkareem
Department of Clinical Nutrition, College of Applied Medical Sciences, University of Ha’il, Ha’il 81442, Saudi Arabia
AbdElmoneim O. Elkhalifa
Department of Clinical Nutrition, College of Applied Medical Sciences, University of Ha’il, Ha’il 81442, Saudi Arabia
Pradyumna Jayahanumaiah
Central Manufacturing Technology Institute (CMTI), Tumkur Road, Bangaluru 560022, India
Mohammad Khalid
Department of Pharmacognosy, College of Pharmacy, Prince Sattam bin Abdulaziz University, Al-Kharj 11942, Saudi Arabia
Natchimuthu Balashanmugam
Central Manufacturing Technology Institute (CMTI), Tumkur Road, Bangaluru 560022, India
Inflammatory breast cancer (IBC) is one of the most belligerent types of breast cancer. While various modalities exist in managing/treating IBC, drug delivery using microneedles (MNs) is considered to be the most innovative method of localized delivery of anti-cancer agents. Localized drug delivery helps to treat IBC could limit their adverse reactions. MNs are nothing but small needle like structures that cause little or no pain at the site of administration for drug delivery via layers of the skin. The polyethylene glycol diacrylate (PEGDA) based MNs were fabricated by using three dimensional (3D) technology called Projection Micro-Stereo Lithography (PµSL). The fabricated microneedle patches (MNPs) were characterized and coated with a coating formulation comprising of gemcitabine and sodium carboxymethyl cellulose by a novel and inventive screen plate method. The drug coated MNPs were characterized by various instrumental methods of analysis and release profile studies were carried out using Franz diffusion cell. Coat-and-poke strategy was employed in administering the drug coated MNPs. Overall, the methods employed in the present study not only help in obtaining MNPs with accurate dimensions but also help in obtaining uniformly drug coated MNPs of gemcitabine for treatment of IBC. Most importantly, 100% drug release was achieved within the first one hour only.
