Nature Communications (Jul 2019)

Pooled clone collections by multiplexed CRISPR-Cas12a-assisted gene tagging in yeast

  • Benjamin C. Buchmuller,
  • Konrad Herbst,
  • Matthias Meurer,
  • Daniel Kirrmaier,
  • Ehud Sass,
  • Emmanuel D. Levy,
  • Michael Knop

DOI
https://doi.org/10.1038/s41467-019-10816-7
Journal volume & issue
Vol. 10, no. 1
pp. 1 – 13

Abstract

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Construction of yeast libraries is time-consuming, costly and limited to the genetic background of the chosen strain. Here the authors present CASTLING which uses CRISPR-Cas12a and oligonucleotide pools to rapidly generate pooled libraries with large insertions such as fluorescent protein tags.