Nature Communications (Jul 2019)
Pooled clone collections by multiplexed CRISPR-Cas12a-assisted gene tagging in yeast
Abstract
Construction of yeast libraries is time-consuming, costly and limited to the genetic background of the chosen strain. Here the authors present CASTLING which uses CRISPR-Cas12a and oligonucleotide pools to rapidly generate pooled libraries with large insertions such as fluorescent protein tags.