Nature Communications (Aug 2016)

Efficient ex vivo analysis of CD4+ T-cell responses using combinatorial HLA class II tetramer staining

  • Hannes Uchtenhagen,
  • Cliff Rims,
  • Gabriele Blahnik,
  • I-Ting Chow,
  • William W. Kwok,
  • Jane H. Buckner,
  • Eddie A. James

DOI
https://doi.org/10.1038/ncomms12614
Journal volume & issue
Vol. 7, no. 1
pp. 1 – 12

Abstract

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Detection of antigen specificity in a blood sample is limited by low frequencies of individual clones. Here the authors develop an MHC class II combinatorial technique and apply it to immunophenotype human CD4+ T cells of multiple specificities in response to influenza vaccine in single blood samples.