Nature Communications (Aug 2016)
Efficient ex vivo analysis of CD4+ T-cell responses using combinatorial HLA class II tetramer staining
Abstract
Detection of antigen specificity in a blood sample is limited by low frequencies of individual clones. Here the authors develop an MHC class II combinatorial technique and apply it to immunophenotype human CD4+ T cells of multiple specificities in response to influenza vaccine in single blood samples.