Cytotoxicity of amorphous calcium phosphate multifunctional composite coatings on titanium obtained by in situ anodization/anaphoretic deposition

Abstract

The cytotoxicity of amorphous calcium phosphate (ACP) and chitosan lactate (ChOL) multifunctional and hybrid composite coatings on MRC-5 human lung fibroblast cell line was elucidated. ACP/TiO2 and ACP/TiO2/ /ChOL were deposited onto Ti by a novel in situ anodization/anaphoretic process at constant voltage. Cytotoxicity tests showed that there was no significant decrease in the survival of healthy MRC-5 cells exposed to composite samples without chitosan lactate, while there was an increase in the number of viable cells in the sample containing ChOL. These findings show that there was improved cell proliferation, differentiation and cell viability in the ChOL-containing sample, which makes ACP/TiO2/ChOL coating a good candidate for applications in medicine and stomatology.

Keywords

• cytotoxicity
• dye exclusion test
• colorimetric test with tetrazolium salts
• amorphous calcium phosphate
• chitosan oligolactate