Nature Communications (Dec 2019)
Extracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid culture
- Giovanni Giuseppe Giobbe,
- Claire Crowley,
- Camilla Luni,
- Sara Campinoti,
- Moustafa Khedr,
- Kai Kretzschmar,
- Martina Maria De Santis,
- Elisa Zambaiti,
- Federica Michielin,
- Laween Meran,
- Qianjiang Hu,
- Gijs van Son,
- Luca Urbani,
- Anna Manfredi,
- Monica Giomo,
- Simon Eaton,
- Davide Cacchiarelli,
- Vivian S. W. Li,
- Hans Clevers,
- Paola Bonfanti,
- Nicola Elvassore,
- Paolo De Coppi
Affiliations
- Giovanni Giuseppe Giobbe
- Stem Cell and Regenerative Medicine Section, University College London GOS Institute of Child Health
- Claire Crowley
- Stem Cell and Regenerative Medicine Section, University College London GOS Institute of Child Health
- Camilla Luni
- Shanghai Institute for Advanced Immunochemical Studies (SIAIS), ShanghaiTech University
- Sara Campinoti
- Stem Cell and Regenerative Medicine Section, University College London GOS Institute of Child Health
- Moustafa Khedr
- Stem Cell and Regenerative Medicine Section, University College London GOS Institute of Child Health
- Kai Kretzschmar
- Oncode Institute, Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW) and University Medical Center (UMC) Utrecht
- Martina Maria De Santis
- Stem Cell and Regenerative Medicine Section, University College London GOS Institute of Child Health
- Elisa Zambaiti
- Stem Cell and Regenerative Medicine Section, University College London GOS Institute of Child Health
- Federica Michielin
- Stem Cell and Regenerative Medicine Section, University College London GOS Institute of Child Health
- Laween Meran
- Stem Cell and Regenerative Medicine Section, University College London GOS Institute of Child Health
- Qianjiang Hu
- Shanghai Institute for Advanced Immunochemical Studies (SIAIS), ShanghaiTech University
- Gijs van Son
- Oncode Institute, Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW) and University Medical Center (UMC) Utrecht
- Luca Urbani
- Stem Cell and Regenerative Medicine Section, University College London GOS Institute of Child Health
- Anna Manfredi
- Telethon Institute of Genetics and Medicine (TIGEM)
- Monica Giomo
- Veneto Institute of Molecular Medicine & Dept. of Industrial Engineering, University of Padova
- Simon Eaton
- Stem Cell and Regenerative Medicine Section, University College London GOS Institute of Child Health
- Davide Cacchiarelli
- Telethon Institute of Genetics and Medicine (TIGEM)
- Vivian S. W. Li
- Stem Cell and Cancer Biology Lab, the Francis Crick Institute
- Hans Clevers
- Oncode Institute, Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW) and University Medical Center (UMC) Utrecht
- Paola Bonfanti
- Stem Cell and Regenerative Medicine Section, University College London GOS Institute of Child Health
- Nicola Elvassore
- Stem Cell and Regenerative Medicine Section, University College London GOS Institute of Child Health
- Paolo De Coppi
- Stem Cell and Regenerative Medicine Section, University College London GOS Institute of Child Health
- DOI
- https://doi.org/10.1038/s41467-019-13605-4
- Journal volume & issue
-
Vol. 10,
no. 1
pp. 1 – 14
Abstract
Organoid cultures have been developed from multiple tissues, opening new possibilities for regenerative medicine. Here the authors demonstrate the derivation of GMP-compliant hydrogels from decellularized porcine small intestine which support formation and growth of human gastric, liver, pancreatic and small intestinal organoids.
